紫珠叶总黄酮提取和大孔树脂纯化的工艺研究

Extraction and purification of total flavonoids from folium callicarpae pedunculatae with macroporous resin

目的大孔树脂是近年来发展起来的一类有较好吸附性能的高聚物吸附剂,可广泛应用于有效成分的分离纯化,而目前国内外对紫珠叶总黄酮的研究较少,文中对紫珠叶总黄酮提取及大孔树脂纯化的工艺进行研究,以提高紫珠叶粗提物中总黄酮含量。方法采用正交试验法,以总黄酮含量为指标优化提取工艺;筛选出吸附及解吸效果好的树脂进行研究,以总黄酮转移率、纯度为指标考察优选树脂的纯化工艺。结果紫珠叶总黄酮的最佳提取工艺为:加20倍量70%的乙醇,回流提取3次,每次1.5 h;AB-8树脂对紫珠叶总黄酮的最佳纯化工艺为:上样液浓度3.77mg/m l,吸附速率1 BV/h,洗脱溶剂为70%乙醇,洗脱流速2 BV/h,洗脱剂用量为4 BV。结论紫珠叶总黄酮提取物经AB-8树脂纯化后,总黄酮纯度不低于57%,该提取精制路线达到富集有效成分的目的。

Objective Macroporous resin,a polymeric sorbent with a good adsorption property developed in recent years,is widely applicable to the separation and purification of active ingredients.Few researches are reported on the total flavonoids from folium callicarpae pedunculatae（FCP） at home and abroad.The authors studied the techniques of the extraction and purification of total flavonoids,aiming at increasing the content of total flavonoids in the crude extracts of FCP.Methods The extraction process was optimized by orthogonal design trial with the content of total flavonoids as the index.Macroporous resin with better adsorbability was selected for this study.And the rates of transfer and purity of total flavonoids were used as the index in the optimization of the purification process.Results The optimum conditions for the extraction of FCP were 20 times the amount of 70% alcohol and three extractions of 1.5 h each,and those for its purification with AB-8 macroporous resin were sample concentration of 3.77 mg/mL,adsorption velocity of 1 BV/h,70% alcohol as the desorption solvent,eluting velocity of 2BV/h,and eluate volume of 4BV.Conclusion After purification with AB-8 macroporous resin,the purity of total flavonoids in the extract of FCP amounted to 57%.This process can be used to effectively separate and concentrate total flavoionds from FCP.