梅花鹿鹿茸尖端组织ESTs分析

Analysis of expressed sequence tags (ESTs) in sika deer (Cervus nippon hortulorum) velvet tip tissue

文章通过对东北梅花鹿(Cervus nippon hortulorum)鹿茸尖端组织cDNA文库随机测序获得了906条高质量ESTs,906条ESTs拼接后代表了701个Unigenes,其中包括重叠群86个,单拷贝615个。Blast分析显示具已知和推测功能的基因580个(82.7%),通过Gene Ontology(GO)分类对获得的580个功能基因进行了包括分子功能、生物过程和细胞组分在内的3个层次的功能注释,并根据BLAST的注释结果及进一步的筛选与分析,共得到39条与鹿茸尖端组织生长发育相关的基因。cDNA文库的构建和ESTs分析填补了鹿科动物在NCBI公共数据库上基因组信息的空白,并为科学的开发和利用梅花鹿资源提供了重要的理论依据。

A total of 906 high quality ESTs were obtained through random sequencing from velvet tip tissue cDNA library of Sika Deer （Cervus nippon hortulorum）. Clustering of 906 ESTs resulted in a set of 701 unigenes comprising of 86 contigs and 615 singletons. BLAST analysis revealed that 580 unigenes （82.7% of all 701 unigenes） were matched to the known genes or putative genes in public databases, which could be classified into molecular function, biological process or cellular component according to the Gene Ontology （GO） classifications. Thirty-nine unigenes were identified to be growth and development-related genes. This study provides the data available for NCBI public database of the deer genome.