吲哚胺2,3-双加氧酶在哮喘小鼠模型中的作用(英文)

The role of indoleamine 2,3-dioxygenase in an animalasthma model

目的探讨吲哚胺2,3-双加氧酶(IDO)在哮喘小鼠模型中所起的作用。方法卵白蛋白(OVA)致敏、激发诱导哮喘小鼠模型。分别检测肺组织中IDO在蛋白水平和mRNA水平上的表达。免疫荧光组织化学方法检测树突状细胞(DCs)在肺组织中的分布和成熟状态。结果①OVA诱导和激发的小鼠哮喘模型的症状和肺部炎症病理改变较对照组严重;哮喘组小鼠血清总IgE为(165.50±30.13)ng/ml,对照组为(94.45±28.30)ng/ml,差异有统计学意义(P<0.05)。②哮喘组小鼠肺部IDO的表达低于对照组。以平均累积光密度为指标检测的小鼠肺组织中IDO在蛋白水平上的表达,哮喘组为11.38±6.05,对照组为23.62±8.92,差异有统计学意义(P<0.05);实时荧光定量PCR检测小鼠肺组织中IDO在mRNA水平上的表达,哮喘组是对照组的33%,显著低于对照组(P<0.05)。③小鼠肺组织中CD11c+CD86+细胞主要分布于肺泡壁和小血管周围。以双标阳性细胞平均累积荧光强度为指标检测小鼠肺组织中CD11c+CD86+细胞数量,哮喘组的中位数为9 961.86(7 406.52～12 724.98),对照组为15 974.60(10 006.39～16 171.46),差异有统计学意义(P<0.05)。结论哮喘小鼠肺组织表达IDO低,且成熟树突状细胞减少。提示在哮喘小鼠中,由于成熟的树突状细胞较少,产生IDO偏少,这可能在哮喘发生中起着重要作用。

Objective To investigate the role of indoleamine 2,3-dioxygenase（IDO）in asthma.Methods The mouse asthma model was induced by ovalbumin（OVA）.IDO expression was detected on the level of protein and mRNA respectively.Distribution and maturation of dendritic cells were detected by the immunofluorescence method.Results（1）The symptoms and lung inflammation in the model group were more serious than control group.The serum total IgE was significantly higher in the model group than that in the control group,165.50 ± 30.13 ng/ml vs.94.45 ± 28.30 ng/ml（P〈 0.05）.（2）IDO expression in the model group was lower than that in control group.On the level of protein,mean intergrated optical density was 11.38 ± 6.05 in the model group vs.23.62 ± 8.92 in the control group（P〈 0.05）;on the level of mRNA,IDO expression of the model group was 33% of the control group（P〈 0.05）.（3）CD11c＋CD86＋ cells were distributed in alveolar wall and around small vessels.The quantity of CD11c＋CD86＋ cells in lungs of the model group were significantly smaller than that in the control group.The median intergrated fluorescence intensity was 9961.86（range,7 406.52 ～ 12 724.98）in the model group vs.15974.60（range,10 006.39 ～ 16 171.46）in the control group（P 〈0.05）.Conclusions IDO expression is low and matured dendritic cells are less in situ in the asthma model.These suggest that less matured DCs may produce less IDO,which may play an important role in asthma.