不同肾脏血管损害程度的紫癜性肾炎患儿外周血内皮祖细胞数量与功能变化

The number of peripheral blood progenitor cell and function in Henoch-Schnlein purpura nephritis children with different degrees of renal vascular lesions

目的探讨内皮祖细胞(EPCs)数量、迁移与黏附能力的变化及与紫癜性肾炎(HSPN)患儿肾脏血管损害的关系。方法将48例HSPN患儿按肾脏血管损害程度分为轻、中、重3组;分离与培养外周血单个核细胞,免疫荧光法鉴定EPCs,流式细胞术检测EPCs的数量,并检测其迁移与黏附能力。结果血管中、重度病变组外周血CD34、血管内皮生长因子受体2(KDR)、CD133阳性细胞率均低于轻度病变组(P均<0.05),血管重度病变组外周血CD34、KDR、CD133阳性细胞率均低于轻度病变组与中度病变组(P均<0.05)。血管轻、中、重度病变组外周血EPCs黏附与迁移能力依次降低,重度病变组明显高于轻、中度病变组(P均<0.05)。结论肾脏血管损伤参与HSPN的发生与发展,而EPCs的数量、迁移与黏附能力变化是肾脏血管病变的重要影响因素。

Objectives To explore the relationships among different degrees of renal vascular lesions in children with Henoch-Schnlein purpura nephritis（HSPN）and changes of progenitor cell number and function in peripheral blood.Methods Forty-eight HSPN children were divided into three groups,mild,moderate and severe,according to the degree of renal vascular lesions.The peripheral blood mononuclear cells were isolated and cultured.Endothelial progenitor cells（EPCs）were identificated by immunofluorescence assay.The number of EPCs and the migration and adhesion of EPCs were detected by flow cytometry.Results The rate of peripheral blood CD34,KDR,CD133 positive cells were less in severe and moderate vascular lesions groups than that in mild vascular lesions group（all P〈 0.05）.The rate of peripheral blood CD34,KDR,CD133 positive cells were less in severe vascular lesions group than that in mild and moderate vascular lesions groups（all P〈 0.05）.The adhesion and migration of EPCs were reduced in turn in mild,moderate and severe groups.There were significant difference between severe group and mild and moderate groups（all P〈 0.05）.Conclusions Renal vascular lesions involved in the occurrence and development of HSPN,while the number of EPCs,migration and adhesion of of EPCs are the important factors on renal vascular lesions.