血管内皮生长因子多态性与过敏性紫癜的相关性研究

Association of gene polymorphism of vascular endothelial growth factor with clinical manifestations in Henoch-Schnlein purpura children

目的探讨血管内皮生长因子(VEGF)-1154G/A基因的多态性与儿童过敏性紫癜(HSP)的关系。方法取研究对象的颊黏膜细胞,提取DNA,应用聚合酶链反应-限制性内切酶片段长度多态性(PCR-RFLP)技术,对102例HSP患儿进行VEGF-1154G/A基因型分析,102例中包括单纯HSP 50例和合并紫癜性肾炎(HSPN)52例。另选64例年龄、性别相匹配的健康儿童作为对照组。结果病例组(HSP和HSPN)G等位基因频率(81.37%)高于对照组(68.75%),差异有统计学意义(P<0.01)。临床表现肾脏受累型的G等位基因频率高于非肾脏受累型,差异有统计学意义(P<0.01)。HSPN组中表现血尿蛋白尿的病例G等位基因频率与仅表现镜下血尿病例比较,差异有统计学意义(P<0.01)。结论 VEGF-1154G/A基因多态性与儿童HSP的发生以及临床表现肾脏受累有关,G等位基因可能是儿童HSP的易感基因,且与HSPN的发生有关。

Objective To explore the relationship of gene polymorphism of vascular endothelial growth factor（VEGF）-1154G/A with Henoch-Schnlein purpura（HSP）in children.Methods Buccal cells were collected.Polyme-rase chain reaction-restriction fragment length polymorphism（PCR-RFLP）was applied to detect the genotype of VEGF-1154G/A.One hundred and two children with HSP,including 52 children with concurrent nephritis（HSPN）and 50 children without nephritis（HSP）were enrolled.Meanwhile,64 age and sex matched healthy children were enrolled as control group.Results G allele frequency in both HSP and HSPN groups（81.37%）was significantly higher than that in control group（68.75%）（P 〈0.01）.According to the clinical manifestations,G allele frequency in patients with renal involvment was significantly higher than patients with non-renal involvment（P〈 0.01）.In HSPN group,G allele frequency in patients with hematuria and proteinuria was higher than patients with microscopic hematuria（P 0.01）.Conclusions VEGF-1154G/A gene polymorphism maybe associated with the susceptibility of HSP and renal involvement.G allele maybe a susceptible gene of HSPN.