还原型谷胱甘肽对大鼠深低温暂停循环的脑保护作用

Protective effect of reduced glutathione on brain with deep hypothermia circulatory arrest in rats

目的探讨还原型谷胱甘肽(GSH)对深低温暂停循环(DHCA)大鼠是否具有脑保护作用。方法 SD大鼠24只,随机分为3组,每组8只,A组:常温体外循环(CPB)组;B组:DHCA组;C组:GSH处理组。各组大鼠转流前及转流结束后30 min留取静脉血检测S-100β及神经元特异性烯醇化酶(NSE)。术后留取脑组织,用于测定脑含水量及病理变化。结果 DHCA组病理变化较常温CPB组明显,而与GSH处理组差别不大。转流后DHCA组大鼠脑含水量较常温CPB组显著增加(P=0.012),GSH处理组大鼠脑含水量与常温CPB组差异无统计学意义。各组转流后S-100β和NSE均较术前增加,DHCA组增加最为明显,但3组间差异无统计学意义。结论 DHCA对大鼠的脑损伤严重;GSH预处理对施行DHCA的大鼠可能有一定的脑保护作用。

Objectives To explore the protective effect of reduced glutathione（GSH）on brain with deep hyperthermia circulatory arrest（DHCA）in rats.Methods Twenty-four male Sprague-Dawley rats were randomly divided into 3 groups,normothermic cardiopulmonary bypass（CPB）group;DHCA group;GSH preconditioning group.Before and after CPB,a venous blood was obained for detection of proteins S-100β and NSE.The brain was removed quickly after the rats had been sacrificed.Some of the brains were used to compute the water content;others were used to detect the pathologic changes.Results DHCA group had a significantly more apparent pathological changes than normothermic CPB group,but GSH preconditioning group did not.After CPB,DHCA group had a significantly higher brain water content than normothermic CPB group（P = 0.012）.GSH preconditioning group had a lower brain water content,but the difference is not significant when compared with normothermic CPB group and DHCA group.After CPB,the level of S-100β and NSE was raised significantly in all groups.Among them,DHCA group had the highest level.However,there were no significant differences between three groups.Conclusions The brain is seriously damaged by DHCA in rats.GSH precondition might have protective effect on brain with DHCA to some extent.