海藻色素糖蛋白对小鼠H22肝癌细胞增殖与凋亡的影响

Effects of seaweed pigment glycoprotein on the proliferation and apoptosis of mice hepatocarcinoma 22 cells

目的:研究麒麟菜海藻色素糖蛋白(seaweed pigment glycoprotein,SPG)对小鼠H22肝癌细胞增殖与凋亡的影响。方法:将不同浓度SPG与小鼠H22肝癌细胞共同培养,用MTT法测定癌细胞增殖活性。建立H22移植瘤小鼠肝癌模型,随机分为SPG高、中、低剂量组,对照组及环磷酰胺组。实验组小鼠每天分别给予不同剂量(100、50、10mg/kg)的SPG灌胃,连续10d,对照组同法给予等量生理盐水,环磷酰胺组小鼠腹腔注射20mg/kg环磷酰胺,隔日一次。各组小鼠均于末次给受试物后24h处死,取肿瘤组织用免疫组化法检测各组Bcl-2和Bax蛋白表达。结果:SPG高剂量组瘤细胞增殖活性(0.545±0.002)明显高于对照组(0.404±0.008)(P<0.05);SPG高剂量组Bcl-2和Bax蛋白阳性表达率分别为16.78%和38.1%,对照组分别为65.16%和4.68%,两组间的差异具有统计学意义(P<0.05)。结论:SPG具有抑制小鼠H22肝癌细胞增殖、促进其凋亡的作用。

OBJECTIVE：To study the effects of SPG on the proliferation and apoptosis of mice hepatocarcinoma 22 cells.METHODS：Different concentrations of SPG were cultured with hepatocarcinoma 22（H22） cells,MTT assay was used to analyze the cell proliferation.The H22-bearing mice were randomly divided into SPG high,medium,low dose groups（100,50,10 mg/kg）,tumor control group and the cyclophosphamide group.Mice in exprimental groups were given daily by gavage with different doses of SPG for 10 days,the control group was given nomal saline and mice in cycliphosphamide group were given 20 mg/kg cyclophosphamide by injection every other day.All the mice were sacrificed 24 hours later after the last administration,the levels of the Bcl-2 and Bax protein expression were measured by immunohistochemistry method.RESULTS：The proliferation activity of tumor cells in high-dose SPG group（0.545±0.002） was significantly higher than tumor control group（0.404±0.008）（P〈0.05）.The expression levels of Bcl-2 and Bax protein in high-dose SPG group were 16.78%and 38.1%,respectively,and those of the tumor control group were 65.16% and 4.68%,respectively.The differences between two groups were significant（P〈0.05）.CONCLUSION：SPG could inhibit proliferation and induce apoptosis of H22 hepatocarcinoma cells effectively.