CT引导下套管针种植法制作兔双灶肝癌模型的评价

Preparation of bifocal hepatic VX2 tumor model in experimental rabbits by using CT-guided trochar implantation technique

目的探讨CT引导下经皮套管针种植法制作兔双灶肝癌模型的可行性。方法将30只健康新西兰大耳白兔随机分为2组,种植VX2瘤细胞株。经皮穿刺种植组采用CT引导下经套管针穿刺种植法,开腹组采用传统的开腹种植法,于兔肝左、右叶各种植1处肿瘤,制成双灶肝癌模型。2周后进行MRI扫描,对比两组单、双灶成瘤率、成瘤满意度、肿瘤体积、种植肿瘤的操作时间、动物死亡率及腹腔种植转移率。结果单灶成瘤率穿刺组为80.0%,开腹组83.3%,两组间差异无统计学意义(P>0.05);双灶成瘤率和成瘤满意度为穿刺组73.3%,开腹组75.0%,两组间差异无统计学意义(P>0.05);肿瘤种植满意度穿刺组80.0%,开腹组33.3%,两组间差异有统计学意义(P<0.05);肿瘤体积在经皮穿刺组为(1 695±970)mm3,开腹组为(1 790±1 080)mm3,两组间差异无统计学意义(P>0.05);平均操作时间为穿刺组为(14.6±7.3)min,开腹组(37.3±21.7)min(P<0.05);实际动物死亡率在经皮穿刺组为0,开腹组为20.0%(P>0.05);腹腔种植转移率在穿刺组为20.0%,开腹组为0,两组间差异无统计学意义(P>0.05)。结论 CT引导下经皮套管针种植法制作兔双灶肝癌模型成瘤率高,模型的满意度高,操作简便、省时,尽管腹腔种植转移发生率稍高,仍是一种较好的、可以替代开腹种植肿瘤的方法。

Objective To discuss the feasibility of CT-guided tumor cell implantation through trochar to establish bifocal hepatic VX2 tumor model in experimental rabbits.Methods Thirty healthy New Zealand rabbits were randomly divided into two groups： percutaneous puncture group and laparotomy group.CT-guided tumor cell implantation through trochar was carried out for the rabbits in percutaneous puncture group,while conventional tumor cell implantation was performed for the rabbits in laparotomy group.Tumor cells were implanted into both right and left hepatic lobes to create bifocal hepatic VX2 tumor model in experimental rabbits.MRI was performed two weeks after the procedure.The unifocal and bifocal tumor formation rate,tumor satisfaction rate,tumor volume,operating time,death rate and abdominal implantation metastasis rate were observed,the results were compared between two groups.Results The unifocal tumor formation rate for percutaneous puncture group and laparotomy group was 80.0% and 83.3% respectively（P 0.05）.The bifocal tumor formation rate for percutaneous puncture group and laparotomy group was 73.3% and 75% respectively（P 0.05）.The tumor satisfaction rate for percutaneous puncture group and laparotomy group was 80.0% and 33.3% respectively（P 0.05）.The operation time was shorter in percutaneous puncture group than in laparotomy group [（14.6 ± 7.3） min vs.（37.3 ± 21.7） min,P 0.05].The tumor volume for percutaneous puncture group and laparotomy group was（1 695 ± 970） mm3 and（1 790 ± 1 080） mm3 respectively（P 0.05）.No significant difference in death rate existed between two groups（0% vs.20%,P 0.05）.The abdominal implantation metastasis rate for percutaneous puncture group and laparotomy group was 20% and 0% respectively（P 0.05）.Conclusion In preparing bifocal hepatic VX2 tumor model in experimental rabbits,CT-guided tumor cell implantation through trochar can provide higher tumor formation rate as well as tumor satisfaction rate.This technique is time-saving and easily-manipulated.It can be used as an alternative for conventional laparotomy implantation method although it carries higher abdominal implantation metastasis rate.