钛纳米管表面RGD修饰工艺的初步研究

Optimization Process of RGD Surface Modification on Titanium Nanotubes

目的:通过在TiO2纳米管表面构建RGD(Arg—Gly—Asp)活性肽阵列,掌握钛纳米管表面制备活性肽阵列的工艺方法。方法:通过阳极氧化法在纯钛表面制备TiO2纳米管阵列,通过扫描电镜、原子力显微镜和荧光显微镜研究化学偶联法将RGD组装在钛纳米管表面的工艺。结果:采用3-氨丙基三乙氧基硅烷(APTES)对钛纳米管预处理2h,然后用交联剂N-琥珀酰亚胺3-马来酸亚胺丙酸酯(SMP)连接钛纳米管上的氨基和RGDC中的硫醇基将RGDC组装在钛纳米管上。结论:使用化学偶联的方法,可将RGD短肽共价连接钛纳米管表面构成RGD生物活性涂层。

Objective：To learn the technology of RGD peptide coating on anodized TiO2 nanotube layer.Methods：TiO2 nanotube layer was prepared by anodization.SEM,AFM,and fluorescence microscope were used to analyzed the suitable technology of RGD peptide coating on the TiO2 nanotube layer.Results：After 2 h of preprocessing by the solution of（3-aminopropyl）-triethoxysilane（APTES）,the RGD peptide was assembled to TiO2 nanotube layer by N-succinimidyl-3-maleimidoproprionate（SMP）.Conclusion：The chemical coupling methods can be used to link RGD peptide coating to the nanotube layer.