摘要
目的从HA1基因层面探讨2007-2009年广西壮族自治区(广西)甲3流感病毒的变异、进化发展规律,探寻与WHO疫苗推荐株以及上海市、北京市和浙江省等地当年流行株之间的差异。方法提取广西22株2007-2009年甲3流感毒株RNA,并测定其血凝素重链(HA1)区域的核苷酸序列,采用生物信息软件,分析与当年疫苗推荐株及上海、北京和浙江等地在HAI以及HAI抗原决定簇区域的差异,分析进化关系。结果 22株广西甲3流感病毒2007年与2008年比较接近,HA1区核苷酸和氨基酸的同源性均值分别为99.39%和99.08%;而与2009年变异较大,同源性均值分别为99.04%和97.78%。而对比世界卫生组织(WHO)推荐的2008-2009年甲3流感疫苗株A/B risbane/10/2007,广西3年分离株HA1区氨基酸酸同源性均值分别为99.81%、99.25%和97.94%。2009年广西甲3流感病毒与当年疫苗株比较,HA1抗原决定簇发生了6个氨基酸的替换,且发生在A、D、E三区。各年度散发株和暴发株同源性较均在99.00%以上。2007年广西甲3毒株与上海及北京比,HA1区氨基酸同源性均值分别为99.83%和99.12%,2008年比较结果分别为98.93%和99.25%。基因进化树分析显示2009年分离株与2007年、2008年及当年疫苗株距离较远,单独分支。结论广西2007年与2008年甲3流感病毒HA1基因特性较为接近,2009年毒株发生了较大变异。广西与上海、浙江等地分离株更为接近,而与北京分离株差异较大。WHO推荐的当年疫苗株均滞后广西流行株。
Objective To study the genetic variation and evolution characteristics of HA1 gene of influenza A（H3N2） virus isolated in Guangxi from 2007 to 2009 and compare the genetic differences on HA1 regions between A3 virus circulated in Guangxi and vaccine strains recommended by WHO and the strains circulating in Shanghai,Beijing and Zhejiang.Methods Viral RNA of 22 strains isolated in Guangxi during 2007-2009 were extracted and their HA1 genes were amplified and sequenced,and the gene sequences were analyzed with the biometric software.Results The strains isolated in 2007 were closed to the strains isolated in 2008,the homogeneity of nucleotide and amino acids between them was 99.39% and 99.08% respectively,but it was distinct to the strains isolated in 2009,the homogeneity was 99.04% and 97.78% respectively.Compared with vaccine strain A/Brisbane/10/2007 recommended by WHO for 2008-2009,the homogeneity of amino acids was 99.81%,99.25% and 97.94%.The strains isolated in 2009 had replaced 6 amino acids in the antigenic determinant A,D and E.The homogeneities of outbreak strains and sporadic strains were all above 99.00%.The homogeneity of amino acids between the strains isolated in 2007 and the strains in Shanghai and in Beijing was 99.83% and 99.12% respectively,while the homogeneity of amino acids was 98.93% and 99.25% respectively in 2008.The analysis of phylogenetic tree showed that the strains isolated in 2009 was in separate branch,which was far from the strains isolated in 2007-2008 and the vaccine strains recommended by WHO.Conclusion The genetic characteristics of HA1 gene of influenza A（H3N2） virus isolated in Guangxi in 2007 was similar to that isolated in 2008.The strains isolated in 2009 had genetic variation.The strains isolated in Guangxi were closed to the strains isolated in Shanghai and Zhejiang,but distinct to the strains isolated in Beijing.The genetic variation of vaccine strains recommended by WHO was behind the circulating strains in Guangxi.
出处
《疾病监测》
CAS
2011年第4期278-283,共6页
Disease Surveillance
