环磷酰胺、卡铂、表阿霉素、吉西他滨致小鼠血小板及白细胞等降低模型复制方法的探索

The Establishment of Platelet and WBC Decrease Model in Mice with Cyclophosphamide,Carboplatin,Pharmorubicin and Gemcitabine

目的本实验通过探索小鼠血虚模型的复制方法,摸索不同化疗药物复制小鼠血虚模型的有效给药方法和剂量。方法通过多次实验调整给药途径和给药剂量,选择适当的时间测定小鼠血常规,检查模型复制情况,总结有效地给药方法和给药剂量。结果通过多次探索性实验评价出环磷酰胺[1、3]、表阿霉素[2]、卡铂[4]、吉西他滨复制小鼠血小板及白细胞等降低模型的可行性,并摸索得出成功率较高的给药方法和剂量。结论环磷酰胺、表阿霉素、卡铂在适当剂量下复制出小鼠血小板、白细胞降低模型的成功率更高,动物死亡率也较低,适合作为复制此类模型的有效药物;吉西他滨成模率低且动物死亡率较高,不适合作为此类模型复制的药物。

Objective： To explore the method for establish blood deficiency model in rats with different chemotherapeutics. Methods： The routes and doses of medication were adjusted with multiple experiments. The blood routine of the mice was determined at appropriate time, the establishment of the model was examined, and the effective routes and doses of medication were summarized. Results ： Cyclophosphamide, carboplatin, pharmorubicin and gemcitabine were feasible to replicate platelet and WBC decrease model in mice, and the routes and doses with higher successful rate were tracked out. Conclusion： Cyclophosphamide, carboplatin and phannorubicin are effective to establish platelet and WBC decrease model in mice for their higher successful rate with appropriate doses and low mortality of the mice. Gemcitabine is not ap- propriate because of its low successful rate and high mortality.