不同胚胎冷冻方法对冻融胚胎移植患者临床结局的影响

The pregnancy outcomes of different cryopreservation methods of human embryos

目的:比较人卵裂期胚胎玻璃化冷冻和慢速冷冻解冻后胚胎复苏率、卵裂球完整率、种植率以及临床妊娠率,评价两种方法的有效性。方法:选择2009年7月～2010年3月在辅助生殖中心行F-ET的89例患者,将其分为玻璃化冷冻组(46例)和慢速冷冻组(43例),比较解冻后胚胎复苏率、卵裂球完整率、种植率以及临床妊娠率。结果:玻璃化冷冻组与慢速冷冻组比较具有较高的胚胎复苏率(92.7%vs.66.1%)、卵裂球完整率(89.2%vs.61.4%)、种植率(21.1%vs.4.7%)以及临床妊娠率(47.8%vs.23.3%)。结论:玻璃化冷冻法解冻后胚胎复苏率高,卵裂球完整性好,胚胎具有更好地发育潜能,是人卵裂期胚胎冷冻的有效方法。

Objective:To evaluate the efficacy of vitrification and slow freezing for the cryopreservation of human cleavage stage embryos in terms of post-warming survival rate,embryo morphology,implantation rates and clinical pregnancy rates.Methods:The embryos of 89 patients at cleavage stages were cryopreserved either with vitrification(46 patients) or slow freezing(43 patients) methods.After warming,the survival rate,embryo morphology,implantation rates and clinical pregnancy rates were evaluated and compared between the two groups.Results:In the vitrification group versus slow freezing group,the survival rate(92.7% vs.66.1%) and the post-warmed excellent morphology with all blastomeres intact(89.2% vs.61.4%) were higher(P<0.05).In the vitrification group,the implantation rate(21.1% vs.4.7%)and the clinical pregnancy rate(47.8% vs.23.3%)were also higher(P<0.05).Conclusion:Vitrification in contrast to slow freezing is an efficient method for cryopreservation of human cleavage stage embryos.Vitrification provides a higher survival rate,minimal deleterious effects on post-warming embryo morphology and it can improve clinical outcomes.