IFITM1基因在HeLa细胞中的功能研究

Functional research of IFITM1 gene in HeLa cells

目的:研究IFITM1基因转染对子宫颈癌HeLa细胞的影响,以及抑制子宫颈癌HeLa细胞生长的作用。方法:应用脂质体法介导IFITM1基因转染子宫颈HeLa细胞,用RT-PCR方法、免疫荧光法、MTT法及流式细胞术检测IFITM1基因转染HeLa细胞的生物学特性。结果:转染后的HeLa细胞:①RT-PCR检测及激光共聚焦显示重组质粒组中mRNA及蛋白表达含量明显高于对照组及空载体组,而对照组及空载体组蛋白表达无统计学差异。②MTT结果显示重组质粒组细胞存活率均明显低于空载体组及未转染HeLa细胞组,而后两组无统计学差异。③AnnexinV/PI双色流式细胞仪检测表明,重组质粒组的HeLa细胞凋亡水平明显高于空载体组和未转染HeLa细胞组,有统计学差异。结论:IFITM1蛋白与子宫颈鳞癌有着密切的关系,该基因可能是一种潜在的抑制子宫颈鳞癌发生的基因。

Objective： To evaluate the effects of IFITM1 gene transfection on HeLa cells of cervical cancer, and to study its inhib- itory effects on HeLa cells of cervical cancer. Methods： Transfected IFITM1 to HeLa cells of cervical using liposome - mediated gene trans- fer, and the biological characteristics of IFITM1 transfecting HeLa cell were detected by RT - PCR, immunofluorescence, MTT method and flow cytometry. Results ： The transfected HeLa cells ： ①The result of RT - PCR and confocal laser detection showed that the expression of mRNA and protein were significantly higher in recombinant plasmid group than that of control group and empty vector group, there was no significant difference between control group and empty vector group . ② MTT results showed that survival rate of recombinant cells in recom- binant plasmid group were significantly lower than that of empty vector group and non - transfected HeLa cells group, no significant difference between the latter two groups. ③Annexin V/PI double stainning flow cytometry showed that the apoptosis level was significantly higher in recombinant plasmid group than that of empty vector group and non - transfected HeLa cells group, there was a statistically significant difference. Conclusion： IFITMI has a close relationship to squamous carcinoma of the cervix; This gene may be a potential inhibition of squamous carcinoma.