宫颈病变中HPV亚型感染特点及意义

The character and significance of the subtypes of human papillomaviruses in the cervical lesions

目的:探讨宫颈病变中HPV亚型感染特点及意义。方法:收集277例阴道镜活检病例行HPV基因型测定;相应的宫颈细胞行HPV杂交捕获定量(HCⅡ)检测。结果:随着宫颈病变程度加重,HPV一重感染逐步上升,多重感染逐步下降,CINⅠ组与CINⅢ组、鳞癌组比较差异有统计学意义(P<0.05);HPV亚型感染频度前六位依次为16、58、33、52、31、18;HPV分型与HCⅡ均显示随着宫颈病变程度的加重,HPV感染率上升,但两种方法检测结果差异无统计学意义(P>0.05)。结论:HPV亚型检测可以用于高危人群的宫颈病变筛查,可达到早发现、早治疗的目的,并且明确HPV亚型的分布特点,有助于HPV疫苗的开发应用。

Objective： To investigate the character and significance of the subtypes of human papillomaviruses in the cervical le- sions. Methods ： The genotypes of the HPVs in the samples of 277 cases were collected and measured by colposcopic biopsy, the HPVs in the cervical cells were analyzed and quantitatively measured by using the Hybrid Capture Ⅱ. Results： The single infection of the HPV increased as the cervical lesions became more seriously, but the multi - infections decreased. The difference between CIN Ⅰ and CIN Ⅲ and squamous cell carcinoma were statistically significant （ P 〈 0.05 ） . The most common subtypes of the HPVs were type 16, type 58, type 33, type 52, type 31 and type 18. Both the classification of the HPVs and the detective of the Hybrid Capture Ⅱ demonstrated that the infection rate of the HPVs increased as the cervical lesions became seriously, but the difference between the two detective methods was not statistically significant （ P 〉 0. 05 ） . Conclusion ： The detectives of the HPV subtypes can be used in scanning the cervical lesions of the high risk population for the early diagnosis and management. The understanding of the distribution of the subtypes of the HPVs will aid the research and development of the vaccines of the human papilloma viruses.