SB203580对受照小鼠免疫细胞的作用研究

Effects of SB203580 on the immune cells of irradiated mice

目的:探讨p38抑制剂SB203580(SB)在免疫细胞辐射损伤中的作用。方法:将C57BL/6小鼠分为对照组、照射组、SB组。对照组接受假照射,对其余两组进行4G y全身照射。SB203580 15m g/kg腹腔注射,照射24h后开始给药,隔日给药共给药5次。SB末次给药24h后处死小鼠,取外周血进行分型CD4、CD8、B220检测,取骨髓测定ROS。结果:SB组与照射组比较,外周血白细胞增加,骨髓ROS水平与照射组比较有所下降(P<0.05)。结论:p38抑制剂能降低受照小鼠骨髓细胞的ROS水平,对4G y照射后的外周血无显著保护作用。

Objective： To observe the effects of p38 inhibitor SB203580（SB） on the immune cells in the radiated mice.Methods： C57BL/6 mice were divided into control group,irradiated group and SB group. The control group received sham irradiation,and the rest accepted 4Gy total body irradiation,dose rate 0.8Gy/min.SB was intraperitoneally injected 15mg/kg at1,3,5,7,9 day after irradiation.After 4Gy irradiation 10 days,the peripheral blood was collected by eyeballs enucleation.Then the immune cells phenotypes in peripheral blood was analyzed by flowcytometry.Results： Compared to irradiation group,WBC numbers increased and ROS levels of bone marrow cells decreased in the SB group（P0.05）.Conclusion： The p38 inhibitor SB can decrease the ROS levels of bone marrow cells,has no obvious protective effects on the WBC after 4Gy irradiation.