山仙颗粒对S180肉瘤瘤株转染肠胃功能紊乱小鼠SOD和MDA的影响

The effects of SXG on the expression of SOD and MDA of gastrointestial dysfunciton mice transfected by S180 sarcoma cell

目的:探讨山仙颗粒对S180肉瘤瘤株转染的肠胃能紊乱小鼠SOD和MDA的影响,以及抗恶性肿瘤的作用机制。方法:将50只小鼠先造成肠胃能紊乱模型,取其中40只接种瘤细胞悬液,接种瘤细胞悬液24h后开始给药,接种后第10天处死小鼠,采用物理和生物化学的方法计算抑瘤率、胸腺指数及血清中的SOD活性和MDA水平。结果:山仙颗粒、5-FU及联合用药均有明显的抑瘤作用,抑瘤率分别为29.32%、31.49%和46.27%。5-FU明显降低胸腺指数,山仙颗粒、联合用药对胸腺指数无较大影响,说明5-FU抑制机体免疫功能,山仙颗粒则有提高机体免疫系统功能的作用。山仙颗粒、5-FU对SOD活性和MDA含量的影响均比荷瘤对照组有显著性差异(P<0.05),两药合用效果更明显。结论:山仙颗粒与5-FU均能提高体内SOD活性和降低MDA含量,两药联合应用对小鼠体内SOD水平及MDA含量的影响均优于两种药物的单独使用。

Objective：To Study the effects of SXG resisting the spontaneous metastasis of S180 sarcoma cell on gastrointestial dysfunction mice,and the effects of SXG to SOD and MDA in these mice.Methods：50 mice were made model of gastrointestial dysfunction mice at first,and next,40 mice were made into tumor model groups.24 hours after made tumor model,we gave the mice different medicine.10 days later,we put these mice to death and calculated the rate of tumor inhibition,thymus index,and serum SOD activity,serum MDA content by physical and biochemical methods.Results： SXG and 5-FU and SXG-5-FU could inhibite tumor to grown significantly,the rates of tumor inhibition were 29.32%、31.49% and 46.27%.5-FU could decrease thymus index significantly,but SXG and SXG-5-FU had no effect on thymus index.That is to say,5FU could decrease immunostimulation,but SXG and SXG-5-FU could heighten immunostimulation.To SOD activity and MDA content,the effect of SXG and 5-FU in contrast of these of tumor group,the meaning was prominent and SXG almost hadn＇t difference with 5-FU.SXG and 5-FU were used togather,the effect was more prominent.Conclusion：SXG and 5-FU can heighten the lever of SOD and decrease the content of MDA.when they are used together,the effects to SOD and MDA are better than they are used alone.