摘要
目的:探讨卡培他滨节律化疗对胃癌肿瘤血管生成的影响,并观察其抑瘤效果。方法:建立裸鼠人胃癌移植瘤模型,观察肿瘤生长情况和毒副作用。采用实时定量PCR测定肿瘤组织中血管内皮生长因子(VEGF)mRNA的表达水平,用免疫组织化学染色测定CD31和VEGF的表达。结果:体内试验证实低剂量卡培他滨明显抑制了肿瘤的生长,毒副作用小,且肿瘤组织内的微血管密度(MVD)及VEGF表达均下降。结论:卡培他滨节律化疗能够有效抑制裸鼠胃癌移植瘤的生长及微血管的形成,且无明显的毒副作用。
Objective: To evaluate the anti-angiogenic and anti-tumor effects of low-dose capecitabine on gastric cancer.Methods: BGC-823 human gastric cancer xenograft model was used,and tumor growth and side effects were closely monitored.Quantitative real-time PCR was used to determine vascular endothelial growth factor(VEGF) mRNA levels.Expression of microvessel density(MVD) and VEGF was detected by imunohistochemical staining.Results: Low-dose capecitabine significantly inhibited BGC-823 tumor growth in the absence of toxicity,which was accompanied by decreases in MVD and VEGF gene expression in tumour tissues.Conclusion: Low-dose capecitabine treatment is effective against gastric tumour and can inhibit the formation of microvessels without toxic effect on nude mice.
出处
《陕西医学杂志》
CAS
2011年第5期529-531,536,共4页
Shaanxi Medical Journal
基金
国家自然科学基金(No30973503
81071650和81050007)
2009年辽宁省高校攀登学者支持计划(2009-2011)
2009年度高等学校博士学科点专项科研基金(20092104110008)
沈阳市科学技术计划项目(F10-149-9-45)
关键词
胃肿瘤/病理学
胃肿瘤/药物疗法
血管生成因子/代谢
抗肿瘤药/治疗应用
内皮
血管
Stomach neoplasms/pathology Stomach neoplasms/drug therapy Angiogenesis factor/metabolism Antineoplastic agents/therapeutic use Endothelium
vascular