卡培他滨节律化疗对胃癌肿瘤血管生成的影响

Antiangiogenic effect of metronomic capecitabine chemotherapy on gastric cancer

目的:探讨卡培他滨节律化疗对胃癌肿瘤血管生成的影响,并观察其抑瘤效果。方法:建立裸鼠人胃癌移植瘤模型,观察肿瘤生长情况和毒副作用。采用实时定量PCR测定肿瘤组织中血管内皮生长因子(VEGF)mRNA的表达水平,用免疫组织化学染色测定CD31和VEGF的表达。结果:体内试验证实低剂量卡培他滨明显抑制了肿瘤的生长,毒副作用小,且肿瘤组织内的微血管密度(MVD)及VEGF表达均下降。结论:卡培他滨节律化疗能够有效抑制裸鼠胃癌移植瘤的生长及微血管的形成,且无明显的毒副作用。

Objective： To evaluate the anti-angiogenic and anti-tumor effects of low-dose capecitabine on gastric cancer.Methods： BGC-823 human gastric cancer xenograft model was used,and tumor growth and side effects were closely monitored.Quantitative real-time PCR was used to determine vascular endothelial growth factor（VEGF） mRNA levels.Expression of microvessel density（MVD） and VEGF was detected by imunohistochemical staining.Results： Low-dose capecitabine significantly inhibited BGC-823 tumor growth in the absence of toxicity,which was accompanied by decreases in MVD and VEGF gene expression in tumour tissues.Conclusion： Low-dose capecitabine treatment is effective against gastric tumour and can inhibit the formation of microvessels without toxic effect on nude mice.