不同针刺法对大鼠腓肠肌砸伤后微循环血流灌注的影响

Effect of different acupuncture therapy on microvascular perfusion after blunt skeletal muscle injury in rats

目的应用激光多普勒灌注成像仪观察不同针刺法对大鼠肌肉组织损伤后微循环障碍的影响,为选择针刺疗法提供实验依据。方法 30只雄性Wistar大鼠随机分为正常对照组、自然恢复组、内热针组、艾灸加热银质针组、银质针无热组5组,每组6只,后4组经钝性砸伤左侧腓肠肌。15d后对后三组采用三种不同的针刺方法进行干预,观察其微循环改变,并与其他两组大鼠进行对比。结果比较5组大鼠腓肠肌微循环血流灌注情况,自然恢复与正常对照组相比,差异有统计学意义(P<0.05);内热针组和艾灸加热银质针组与正常组比较,差异无统计学意义(P>0.05)。银质针无热组与正常对照组相比,差异有统计学意义(P<0.05)。结论针刺疗法对大鼠损伤的腓肠肌微循环有影响,其中加热的两种方法具有促进微循环恢复的作用。

Objective To observe the effect of different acupuncture therapy on microvascular perfusion after blunt skeletal muscle injury in rats using laser-Doppler perfusion imaging.Methods Thirty male Wistar rats were randomly divided into normal control group,spontaneous recovery group,calor internus acupuncture group,silver acupuncture plus heating group,and silver acupuncture not plus heating group,6 rats in each group.The left gastrocnemius muscle was blunt injured in the last 4 groups.The blunt injuries in the last 3 groups were treated with different acupuncture therapy after 15 days.Changes in microcirculation of the left gastrocnemius muscle were observed and recorded,and compared with those in the other two groups.Results A significant difference was observed in microcirculation between the 5 groups and normal control group（P〈0.05）.No significant difference was found between the calor internus acupuncture group and silver acupuncture plus heating group,while a difference was observed between the silver acupuncture not plus heating group and normal control group（P〈0.05）.Conclusion Acupuncture therapy is effective for microvascular perfusion failure after blunt skeletal muscle injury in rats.