草珊瑚药材毛细管电泳指纹图谱条件探索

Study on Fingerprint Condition of Capillary Electrophoresis in Sarcandra

目的:本试验对草珊瑚药材指纹图谱电泳条件进行摸索。方法:主要对电泳缓冲液的组成,缓冲液pH值,分离电压,分离温度,进样量和添加剂进行考察。结果:草珊瑚药材毛细管指纹图谱的电泳条件为:电泳缓冲液7.5 mmol/L硼砂-磷酸二氢钠-8%乙腈-0.8 mmol/Lβ-环糊精(β-CD),进样量2.76 kPa(3s),分离电压17kV,柱温15℃,检测波长340 nm。结论:通过对电泳条件的全面摸索,得到草珊瑚药材毛细管电泳指纹图谱的最佳分离条件。

Objective：To study on fingerprint condition of capillary electrophoresis（CE） in Sarcandra.Method：Study on following effect factors：composite of buffer,the pH of buffer,division voltage,division temperature,injection volume and additive.Results：The buffer was 7.5 mmol·L-1 borat and sodium dihydrogen phosphate solution containing 8% acetonitrile and 0.8 mmol·L-1 β-CD.The pH of the buffer was adjusted 8.39 with HCl solution.The detection wavelength was 340 nm and a voltage of 17 kV was applied.The sample hydrodynamic injection was 2.76kPa with a duration time of 3 sec.Conclusion：The best fingerprint condition of CE in Sarcandra could be established.