茶籽壳酸水解制备木糖工艺研究

Production of Xylose from Tea Seed Shells with Acid Hydrolysis

以超声波为辅助手段,稀盐酸为催化剂,常压水解茶籽壳制备木糖,利用3,5-二硝基水杨酸比色法(DNS法)对水解液中的木糖进行检测。通过正交试验考察了超声预提时间、酸浓度、茶籽壳粉碎度、水解时间等对木糖产率的影响。研究表明各因素对木糖得率的影响由大到小的顺序依次为水解时间、酸浓度、粉碎度、超声预提时间;最佳制备条件为:超声预提时间为45 min,功率为50 kHz,温度为25～30℃;酸浓度为0.6 mol/L;粉碎度为40目;水解时间为3 h。在此条件下,木糖产率最高,达28.44%。研究为茶籽壳的利用提供了一种简便易行的参考方法。

In order to produce xylose from tea seed shells,The method of hydrolysis of tea seed shells under preeure was used,and the dilute hydrochloric acid as a catalyst,and the ultrasonic waveas the assisted method.The xylose was monitored by 3,5-dinitrosalicylic acid（DNS） colorimetric method.The hydrolytic factors including pre-extraction time,hydrochloric acid concentration,hydrolysis time were investigated by means of orthogonal test.Results have demonstrated that the importance of various factors were arranged in following order： time of hydrolysisconcentration of acidparticlesizetime of ultrasonic wave pre-extration.The optimum factors production were as follows： 45 min,ultra-sonic wave pre-extration,ultrasonic frequency of 50 kHz at 25～30℃,hydrochloric acid concentration at 0.6 mol/L,particle size of seed shell at 40 mesh hydrolysis time for 3 h.Under the above mentioned conditions,the highest productivity of xylose was obtained,the yield was up to 28.44%.This study provided an simple method on the comprehensive utilization of tea seed shells.