摘要
以超声波为辅助手段,稀盐酸为催化剂,常压水解茶籽壳制备木糖,利用3,5-二硝基水杨酸比色法(DNS法)对水解液中的木糖进行检测。通过正交试验考察了超声预提时间、酸浓度、茶籽壳粉碎度、水解时间等对木糖产率的影响。研究表明各因素对木糖得率的影响由大到小的顺序依次为水解时间、酸浓度、粉碎度、超声预提时间;最佳制备条件为:超声预提时间为45 min,功率为50 kHz,温度为25~30℃;酸浓度为0.6 mol/L;粉碎度为40目;水解时间为3 h。在此条件下,木糖产率最高,达28.44%。研究为茶籽壳的利用提供了一种简便易行的参考方法。
In order to produce xylose from tea seed shells,The method of hydrolysis of tea seed shells under preeure was used,and the dilute hydrochloric acid as a catalyst,and the ultrasonic waveas the assisted method.The xylose was monitored by 3,5-dinitrosalicylic acid(DNS) colorimetric method.The hydrolytic factors including pre-extraction time,hydrochloric acid concentration,hydrolysis time were investigated by means of orthogonal test.Results have demonstrated that the importance of various factors were arranged in following order: time of hydrolysisconcentration of acidparticlesizetime of ultrasonic wave pre-extration.The optimum factors production were as follows: 45 min,ultra-sonic wave pre-extration,ultrasonic frequency of 50 kHz at 25~30℃,hydrochloric acid concentration at 0.6 mol/L,particle size of seed shell at 40 mesh hydrolysis time for 3 h.Under the above mentioned conditions,the highest productivity of xylose was obtained,the yield was up to 28.44%.This study provided an simple method on the comprehensive utilization of tea seed shells.
出处
《茶叶科学》
CAS
CSCD
北大核心
2011年第3期195-200,共6页
Journal of Tea Science
基金
现代农业(茶叶)产业技术体系建设专项资金(农科教发[2008]10号)
安徽省研究实验基地优秀中青年科研带头人专项基金
安徽农业大学人才启动基金资助
关键词
茶籽壳
酸水解
木糖
tea seed shell
acid hydrolysis
xylose