高效液相色谱法测定人血浆中间氯苯哌嗪的浓度

Determination of m-Chlorophenylpiprazine in Human Plasma by HPLC

目的:建立测定人血浆中间氯苯哌嗪浓度的高效液相色谱法。方法:以Diamonsil C_(18)反相柱(150 mm×4.6 mm,5μm)为色谱柱,流动相为0.085 mol·L^(-1)磷酸缓冲液-乙腈-甲醇(82:8:10);流速1 ml·min^(-1);柱温40℃;检测波长250 nm。以乙酸乙酯为提取剂。结果:间氯苯哌嗪线性范围为0.033 6～3.360 0μg·ml^(-1)。间氯苯哌嗪低、中、高(0.077 2,0.252 0,2.240 0μg·ml^(-1))3种浓度平均回收率分别为96.9%,101.9%和97.2%;日内、日间RSD均<7%(n=5)。结论:该方法灵敏、准确、简单、快速,可用于临床血药浓度监测和药动学研究。

Objective： To establish a method for determining m-chlorophenylpiprazine in human plasma by HPLC. Method： The drug in plasma was analyzed in a RP-HPLC system with C18 column （ 150 mm ×4. 6 mm, 5 μm）. The mobile phase consisted of 0. 085 mul·L^-1 phosphate buffer-acetonitrile-methanol （82： 8： 10） with the flow rate of 1 ml· min^-1. The detection wavelength was at 250 nm. Ethyl acetate was used as the extracting solvent. Result： The average recovery for low, medium and high concentration of m-chlo- rophenylpiprazine （0. 077 2,0. 252 0 and 2. 240 0μg·ml^-1 ） was 96. 9%, 101.9% and 97. 2% , respectively. The intra-day and interday variations （RSD） were less than 7% （n = 5 ）. The calibration curve of m-chlorophenylpiprazinc showed good linearity over the range of 0. 033 6-3. 360 0 μg·ml^-1. The regression equation was the following： Y = 156. 33X- 6. 54 （r = 0. 999 1 ）. Conclusion： The method is sensitive, accurate, simple and quick, which can be used tot the clinical drug monitoring and pharmacokinetic studies.