山西产蒲公英药材品质研究

Study on the Quality of Medicinal Material of Herba Taraxaci Produced in Shanxi

目的:采用HPLC法测定山西产蒲公英中咖啡酸与绿原酸的含量。方法:采用十八烷基硅烷键合硅胶为填充剂,以磷酸(含0.1%磷酸的水溶液)与乙腈的体积比(85∶15)为流动相,流速1.0mL/min,检测波长322nm。结果:咖啡酸的线性范围为0.8μg/mL～4.0μg/mL(r=0.9966),绿原酸的线性范围0.4μg/mL～2.0μg/mL(r=0.9997);平均回收率:咖啡酸98.73%,RSD为2.49%,绿原酸98.82%,RSD为2.71%。结论:咖啡酸和绿原酸质量分数为0.074%、0.028%,品质优良。

Objective： To determine the contents of caffeic acid and chlorogenic acid in herba taraxaci produced in Shanxi by using HPLC method. Methods： The octadecylsilane chemically bonded silica was taken as the filler, and the phosphoric acid（he phosphate solution containing 0.1% phosphoric acid）： acetonitrile（85：15）as flowing phase with a flow rate of 1.0 ml/min and a detection wavelength of 322 nm. Results： The linear range of caffeic acid and chlorogenic anid were 0.8 μg/mL ～4.0 μg/mL（r=0.9966）and 0.4 μg/mL～2.0 μg/mL（r=0.9997）respectively; the average recovery of caffeic acid and chlorogenic acid was 98.73 % with RSD of 2.49% and 98.82 % with RSD of 2.71% respectively. Conclusion： The mass fraction of caffeic acid and chlorogenic acid was 0.074% and 0.028% respectively, the quality is good.