原儿茶酸对新生大鼠皮质神经元存活及突起生长的影响

Neurotrophic Effects of Protocatechuic Acid on Neurite Outgrowth and Survival in Cultured Cerebral Cortical Neurons of Newborn Rat

目的:研究原儿茶酸(protocatechuic acid,PCA)对新生大鼠皮质神经元存活及突起生长的影响。方法:体外培养新生大鼠皮质神经元,通过细胞形态学观察、神经元细胞活力检测、培养液中乳酸脱氢酶(LDH)渗漏量的测定和有突起神经元数目及平均突起长度定量分析,观察不同剂量的原儿茶酸对神经元的营养作用。结果:与溶媒对照组比较,原儿茶酸低、中、高剂量(16、32、64μmol/L)组神经元活力增强,LDH渗漏量减少,有突起的神经元数目增多,神经元突起增长,神经网络更发达,并有一定的量效关系。结论:原儿茶酸能促进大鼠皮质神经元的存活及突起生长。

Objective：To study the neurotrophic effects of protocatechuic acid on neurite outgrowth and survival in cultured cerebral cortical neurons.Methods：The newborn rat cerebral cortex neurons were cultured in vitro.The convert phase microscope was used to count the survival neurons with neurites and measure the average length of neurites.MTT and LDH assay were carried out to investigate the effect of PCA on the neuronal viability.Results：Compared with control group,different concentration of PCA could increase the number of survival neurons with neurites and the average length of neurites.MTT and LDH assay showed that PCA promoted neuron survival in a dose-dependent manner.Conclusion：PCA can enhance the survival of rat cortical neurons with neurites and promote the neurite outgrowth of rat cortical neurons.