毛细管电泳法测定麻杏石甘汤中盐酸麻黄碱、盐酸伪麻黄碱和盐酸甲基麻黄碱的含量

Determination of Ephedrine Hydrochloride,Pseudoephedrine Hydrochloride and Methylephedrine Hydrochloride in Maxingshigan Decoction by CE

目的：建立测定麻杏石甘汤中盐酸麻黄碱、盐酸伪麻黄碱和盐酸甲基麻黄碱含量的毛细管电泳法。方法：采用未涂层弹性融硅石英毛细管柱（60 cm×55μm ID,有效长度52 cm）;以60 mmol/L硼砂＋10%（体积分数）甲醇（pH 9.0）为运行缓冲液;分离电压12 kV;重力进样10 s（高度15 cm）;检测波长210 nm;以盐酸小檗碱为内标。结果：盐酸麻黄碱、盐酸伪麻黄碱和盐酸甲基麻黄碱浓度分别在20.0~160.0μg/mL（r=0.9999）、7.5~60.0μg/mL（r=0.9991）、2.0~10.0μg/mL（r=0.9993）内线性关系良好,3种成分的平均加样回收率依次为98.0%、97.0%、97.8%,方法精密度（RSD）依次为2.31%、2.21%、2.00%（n=6）。结论：本方法简便、快速,结果准确可靠,可用于麻杏石甘汤的质量控制。

Objective：To establish the method for determination of ephedrine hydrochloride、pseudoephedrine hydrochloride and methylephedrine hydrochloride in Maxingshigan decoction by capillary electrophoresis.Methods：The separation was performed on a fused silica capillary of 60 cm×55 μm ID（52 cm of effective length）.60 mmol/L Na2B4O7＋10%（V/V） CH3OH（pH 9.0） was selected as the running buffer.The separation voltage was 12 kV.The samples was injected by gravity（10 s,15 cm）.The detection wavelength was 210 nm and berberine hydrochloride was the internal standard.Results：The linear range of determination for ephedrine hydrochloride、pseudoephedrine hydrochloride and methylephedrine hydrochloride were 20.0~160.0 μg/mL（r=0.9999）、7.5~60.0 μg/mL（r=0.9991） and 2.0~10.0 μg/mL（r=0.9993）.The average recoveries were 98.0%、97.0% and 97.8%,the precisions of the method were 2.31%、2.21% and 2.00%（n=6）,respectively.Conclusion：The method is convenient,rapid and accurate for the quality control of Maxingshigan decoction.