摘要
目的：建立胆酸和猪去氧胆酸的含量测定方法。方法：采用HPLC—UV法，色谱柱为Diamonsil—C18（4．6mm×150mm，5μm），流动相为乙腈-水，磷酸（35：65：0．1），检测波长为192nm，柱温25℃，流速1．5mL·min^-1。结果：胆酸、猪去氧胆酸分别在3．154～31．540μg和1．927～19．266μg线性关系良好，相关系数分别为r=0．9995和r=0．9992．平均加样回收率分别为98．98％（RSD为0．42％）和98．29％（RSD为0．61％）。结论：方法准确可靠、重复性好，可用于胆酸和猪去氧胆酸的质量控制。
Objective： An HPLC method was used for determination of cholic acid and hyodeoxycholic acid. Methods： Using HPLC- UV with Diamonsil- C18 column （4. 6 mm×150 mm,5 μm） ,acetonitril-water-phosphoric acid （35： 65： 0.1 ） as the mobile phase;the detection wavelength was 192nm;the temperature of the column was 25℃ ; the flow rate was 1.5 mL ·min^-1. Results： A better linearity and good correlation coefficient were obtained：eholic acid was 3. 154 - 31. 540 μg, r = 0. 999 5 ; hyodeoxyeholie acid was 1. 927 - 19. 266μg, r = 0. 9992. The satisfactory recoveries were obtained： cholic acid was 98.98% , RSD = 0. 42% ; hyodeoxycholic acid was 98.29%, RSD = 0. 61%. Conclusion： The method was accurate, reliable and repeatable. It can be used as the method of quality control for cholic acid and hyodeoxycholic acid.
出处
《中国药品标准》
CAS
2011年第2期91-94,共4页
Drug Standards of China
