摘要
目的:建立胆酸和猪去氧胆酸的含量测定方法。方法:采用HPLC—UV法,色谱柱为Diamonsil—C18(4.6mm×150mm,5μm),流动相为乙腈-水,磷酸(35:65:0.1),检测波长为192nm,柱温25℃,流速1.5mL·min^-1。结果:胆酸、猪去氧胆酸分别在3.154~31.540μg和1.927~19.266μg线性关系良好,相关系数分别为r=0.9995和r=0.9992.平均加样回收率分别为98.98%(RSD为0.42%)和98.29%(RSD为0.61%)。结论:方法准确可靠、重复性好,可用于胆酸和猪去氧胆酸的质量控制。
Objective: An HPLC method was used for determination of cholic acid and hyodeoxycholic acid. Methods: Using HPLC- UV with Diamonsil- C18 column (4. 6 mm×150 mm,5 μm) ,acetonitril-water-phosphoric acid (35: 65: 0.1 ) as the mobile phase;the detection wavelength was 192nm;the temperature of the column was 25℃ ; the flow rate was 1.5 mL ·min^-1. Results: A better linearity and good correlation coefficient were obtained:eholic acid was 3. 154 - 31. 540 μg, r = 0. 999 5 ; hyodeoxyeholie acid was 1. 927 - 19. 266μg, r = 0. 9992. The satisfactory recoveries were obtained: cholic acid was 98.98% , RSD = 0. 42% ; hyodeoxycholic acid was 98.29%, RSD = 0. 61%. Conclusion: The method was accurate, reliable and repeatable. It can be used as the method of quality control for cholic acid and hyodeoxycholic acid.
出处
《中国药品标准》
CAS
2011年第2期91-94,共4页
Drug Standards of China