摘要
利用凝胶内溶质分子向缓冲溶液中扩散的方法，测定了溶菌酶、β－乳球蛋白、卵清蛋白、牛血清白蛋白和γ－球蛋白５种蛋白在聚丙烯酰胺凝胶中的有效扩散系数和分配系数，研究了单体质量浓度、交联度和蛋白分子粒径等因素的影响，讨论了聚丙烯酰胺凝胶中蛋白分子的扩散和分配机制。
The effective diffusion coefficient and partition coefficient of five proteins(LysozymeLactoglobulinovalbuminbovine serum albumin and Globulin)in polyacrylamide gels were measured with the method of proteins in gels diffusing into buffer solution.The effects of mass concentrations of monomer,crosslink ratio and the size of protein were studied individually.The present results indicated that the diffusion and partitioning behavior of protein molecules could not be explained by Ogston's theory.
出处
《化学工业与工程》
CAS
1999年第4期187-192,共6页
Chemical Industry and Engineering
基金
国家自然科学基金
关键词
聚丙烯酰胺
凝胶
蛋白
扩散系数
分配系数
polyacrylamide gel,protein
effective diffusion coefficient,partition coefficient
