艾蒿中黄酮的提取纯化及抑菌实验

Extraction and purification of flavonoid in artemisia argyi and its bacteriostatic inhibition

黄酮类物质具有抗菌、抗炎、抗肿瘤等多种功效。艾蒿中含有丰富的黄酮物质。采用超声波辅助回流提取艾蒿中的黄酮,用分光光度法在510nm处测量吸光度。通过响应面分析,得到最佳工艺参数为温度74℃,超声波功率200W,提取时间92min,乙醇浓度60%,料液比1∶35。在该条件下提取两次,测得提取率为91.5%。用AB-8大孔树脂将其纯化。将纯化后的黄酮进行抑菌试验。采用二倍稀释法,用滤纸片法测量艾蒿中黄酮对大肠杆菌、金黄色葡萄球菌、黑曲霉菌、白曲霉菌的最低抑菌浓度。黄酮对大肠杆菌和金黄色葡萄球菌的最低抑菌浓度为0.25mg/mL,对黑曲霉菌和白曲霉菌的最低抑菌浓度为1mg/mL,从而确定大肠杆菌和金黄色葡萄球菌对黄酮较敏感。

Flavonoids isanti-microbial,anti-inflammatory and anti-tumor.Flavonoids is rich in Artemisia argyi.Ultrasonic refluxing extraction was used to extract flavonoids from Artemisia argyi,Spectrophotometry was used to measure the absorbance at 510nm.By response surface analysis,the optimal parameters were as follows：extraction temperature was 74℃,ultrasonic power was 200W,extraction time was 92min,alcohol concentration was 60%,ratio of liquid to material was 1：35.Extracted twice under this condition,the extraction yield of flavonoid was 91.5%.AB-8 macroporous resin were used to purify flavonoids.Flavonoids were used in antibacterial test.By double dilution method,the filter paper was used to measure the minimum inhibitory concentration of flavonoids against Escherichia coli,Staphylococcus aureus,Aspeigillus niger,and Aspergillus candidus.The minimum inhibitory concentration of flavonoids against Escherichia coli and Staphylococcus aureus was 0.25mg/mL,the minimum inhibitory concentration of flavonoids against Aspeigillus niger and Aspergillus candidus was 1mg/mL.Escherichia coli and Staphylococcus aureus were all sensitive to the flavonoids.