摘要
在单因素试验的基础上,采用Box-Behnken设计对杜衡挥发油超声波协同微波提取工艺中的液料比、微波功率和微波时间3因素的最优化组合进行了定量研究,建立并分析了各因素与得率关系的数学模型。结果表明:最佳的工艺条件为液料比10mL/g,微波功率234W和提取时间131 s。经试验验证,在此条件下得率为8.29%,与理论计算值8.32%基本一致。说明回归模型能较好地预测杜衡挥发油的提取得率。并采用气相色谱-质谱联用技术对杜衡挥发油成分进行了分离鉴定,分离出30种成分,确认了其中21种,采用峰面积归一化法确定了各成分的相对含量。
The influences of solvent to material ratio,microwave power and extraction time on the extraction yield of volatile oil from Asarum forbesii Maxim with the ultrasonic-microwave synergistic extraction(UMSE)technique were studied.Box-Behnken experimental design was based on the single factor experiments.One mathematical model established and analyzed by response surface methodology(RSM)was adequate to describe the relationships between the studied factors and the response of volatile oil extraction yield.Based on the canonical analysis,the optimum extraction conditions were obtained as follows:solvent to material ratio 10mL/g,microwave power 234W and extraction time 131s.Under the optimized extraction conditions,the extraction yield is 8.29%,which is close to the estimated value 8.32% attained by using regression model.This model can well predict the experiment results.Then the chemical components of the volatile oil were examined by GC-MS.Finally 30 kinds of compounds were separated,21 kinds of compounds were identified,and the relative contents of these components were determined by peak area normalization method.
出处
《中国食品添加剂》
CAS
北大核心
2011年第2期87-92,共6页
China Food Additives
基金
徐州工程学院青年项目(XKY2009121)
关键词
超声波协同微波提取
杜衡
挥发油
响应曲面法
GC/MS
ultrasonic-microwave synergistic extraction
Asarum forbesii Maxim
volatile oil
response surface methodology
GC/MS