Metabolite identification and the development of a simultaneous quantification method for wogonin and wogonin-7-O-glucuronide:application to a distribution study in mice liver

代谢产物鉴定及汉黄芩素与汉黄芩素-7-O-葡萄糖醛酸苷的同时测定:小鼠肝脏的分布研究(英文)

Wogonin possesses potent inhibitory activities against cancer cell growth in vitro and in vivo and has attractive safety profiles.A highly sensitive liquid chromatography coupled with tandem mass spectrometry method was developed for the identification of major metabolites in mice liver after intravenous administration of wogonin.Five metabolites were identified and biotransformation pathways were elucidated as well.Furthermore,a method was developed and validated for the simultaneous quantitatively determination of wogonin and wogonin-7-O-glucuronide in mice liver.After liquid-liquid extraction by ethyl acetate,the analytes were separated on a C_（18）column with a mobile phase of methanol-10 mM ammonium acetate water （80：20,v/v）.The detection was operated with negative selected reaction monitoring mode using electrospray ionization technique. The linear response range was 0.2-40μg/g for both wogonin and wogonin-7-O-glucuronide in mice liver.The developed quantification method was suitable for distribution study after intravenous infusion of wogonin injection in animals.

汉黄芩素具有体内外抗肿瘤活性及良好的安全性。本文采用高效液相色谱串联质谱法研究了小鼠静脉注射汉黄芩素后肝脏中的代谢产物,鉴定了五个主要的代谢产物。建立了小鼠肝脏中汉黄芩素及其代谢产物汉黄芩素-7-O-葡萄糖醛酸苷的同时测定方法。肝匀浆经醋酸乙酯提取,在C_(18)色谱柱上,采用甲醇-10 mm醋酸铵(80:20,v/v)为流动相,质谱检测采用负离子选择反应监测模式。肝组织中汉黄芩素与汉黄芩素-7-O-葡萄糖醛酸苷在0.2-40μg/g范围内呈良好线性关系,方法学评价表明本法适用于动物静脉注射汉黄芩素后体内的组织分布研究。