肝素-壳聚糖复合体-羟基磷灰石-米诺环素新型仿生纳米复合材料修复兔胫骨缺损

New bionic composite of heparin-chitosan-hydroxyapatite-minocycline repairs rabbit tibial defects

背景:仿生纳米复合材料具有与自体骨相似的组成和结构,有广泛的应用前景。目的:观察肝素-壳聚糖-羟基磷灰石-米诺环素仿生纳米复合材料修复兔胫骨缺损的效果。方法:取20只健康成年新西兰大白兔,制作胫骨上端15mm×8mm的腔隙性临界性骨缺损。随机数字表分为实验组(n=16)和空白对照组(n=4)。实验组植入课题组研制的肝素-壳聚糖复合体-磷灰石-米诺环素仿生纳米复合骨修复材料新型复合材料,空白对照组不进行干预。分别于植入后2,4,8,12周行大体观察、X射线平片及组织学观察新型骨修复材料的骨修复效果。结果与结论:大体观察显示实验组植入后8周后缺损已经融合,12周时塑形接近正常。X射线平片显示随着时间延长,实验组骨缺损骨痂增多,12周基本愈合,塑形完成,空白对照组未见骨性修复,形成骨不连。组织学观察实验组植入后4周材料开始吸收,8周后降解被新生骨取代,12周完全修复,空白对照组各时间点均由纤维组织充填。提示新型肝素-壳聚糖复合体-羟基磷灰石-米诺环素仿生纳米复合材料能有效促进临界性骨缺损的修复。

BACKGROUND：The bionic nano-composite materials have the similar composition and structure with the autologous bone,thus they are promising to apply.OBJECTIVE：To observe the effect of repairing rabbit tibial defects by a new bionic composite of heparin-chitosan-hydroxyapatite-minocycline.METHODS：Twenty healthy adult New Zealand rabbits were used in this study to establish a 15 mm × 8 mm critical size defect on superior tibial bones,and then divided into experiment group（n=16） and blank control group（n=4） at random.A new bionic composite of heparin-chitosan-hydroxyapatite-minocycline was implants into the segmental bone defects of 16 rabbits in experimental group;No implant was given into the other 5 rabbits in the blank group.Gross observation,X-ray and histological examination were performed to observe the repair of bone defects on two groups.RESULTS AND CONCLUSION：General observations showed the fractured bone healed at 8 weeks postoperation in the experimental group,and the appearance was close to normal at 12 weeks.X-ray showed that bony callus gradually increased when time went by in the experimental group,the fractures almost healed and the moulding finished 12 weeks postoperation.No bone defects were repaired and nonunion occurred in the blank group at 12 weeks postoperation.Histological examination indicated the materials began to be absorbed at 4 weeks postoperation,was reconstructed by new bone at 8 weeks,and completely repaired at 12 weeks in the experimental group.In the control group,bone defects were filled with the fibrous tissues.The new bionic composite of heparin-chitosan-hydroxyapatite-minocycline can repair critical size defect effectively.