纳米级羟基磷灰石/骨形态发生蛋白复合物修复兔桡骨大段缺损及局部血管内皮细胞生长因子的表达

Nano-hydroxyapatite/bone morphogenetic protein compound for repair of segmental radius bone defects of rabbits and local vascular endothelial growth factor expression

背景:研究发现,在小段骨缺损中植入骨或仿生骨组织,坏死组织逐渐被替换,移植骨中会长入有活性的血管肉芽组织,移植骨被吸收,新骨主动形成。但在大段骨缺损,这一过程发生较慢且不完全。目的:观察纳米级羟基磷灰石材料复合骨形态发生蛋白后大段骨缺损修复能力及诱导生成血管能力。方法:制作兔桡骨大段骨缺损模型,抽签随机分2组,选择一侧分别植入纳米级羟基磷灰石/骨形态发生蛋白、纳米级羟基磷灰石修复,均以另一侧为空白对照。植入后6个月行大体观察、X射线、组织形态学检查、组织切片碱性磷酸酶染色、成骨量分析、血管内皮细胞生长因子阳性细胞率及阳性血管数检测。结果与结论:空白对照组基本无骨组织生成。纳米级羟基磷灰石植入后被新生骨组织分割成小块,材料原有结构破坏。纳米级羟基磷灰石/骨形态发生蛋白组较纳米级羟基磷灰石组残存材料更少,材料降解更为彻底。纳米级羟基磷灰石/骨形态发生蛋白组成骨量、血管内皮细胞生长因子表达及血管内皮细胞生长因子阳性血管数目均明显高于纳米级羟基磷灰石组(P<0.001),且血管内皮细胞生长因子表达与血管内皮细胞生长因子阳性血管数目成正比关系。说明纳米级羟基磷灰石与骨形态发生蛋白复合后,骨修复能力进一步增强,诱导血管生成能力明显提高。

BACKGROUND：Previous studies demonstrated that bone or biomimetic bone tissue is implanted into a cantlet of bone defect,necrotic tissue is gradually replaced,the active vascular granulation tissue grow in bone graft,the bone graft is absorbed,and new bone is formed initiatively.However,the process occurs slowly and incomplete in segmental bone defect.OBJECTIVE：To observe the ability of repairing segmental bone defect and induced vasculogenesis with Nano-hydroxyapatite-collagen（NHAC） materials combined with bone morphogenetic protein（BMP）.METHODS：Segmental radius bone defect models were prepared,which were randomly divided into 2 groups by sortition,NHAC/BMP and NHAC was implanted into one side of models to repairing bone defect,respectively,the other side as blank control group.The effects were observed by gross observation,X-ray examination,histomorphological examination,analysis of histological section alkaline phosphatase staining and bone formation content,positive cell rate and the number of positive vessels of vascular endothelial growth factor（VEGF） detection.RESULTS AND CONCLUSION：There was almost no bone tissue formation in blank control group.NHAC was divided into nubble after implantation;the original structure of materials was damaged.The remaining materials in NHAC/BMP group were less than that in NHAC group,the degradation of materials was more thorough.Bone formation content,VEGF expression,and the number of positive vessels of VEGF of NHAC/BMP in blank control group were higher than that in NHAC group（P 0.001）.VEGF expression was proportional relationship with the number of positive vessels of VEGF.After NHAC combined with BMP,the repair capacity of bone was further reinforced,and the ability of induced angiogenesis was significantly improved.