玻璃化法保存同种异体肌腱移植材料的可行性

Feasibility of flexor tendon allograft cryopreserved with vitrification

背景:处理同种异体肌腱最主要目的是减少免疫原性和保持肌腱结构与活性。现在临床上常用的低温冷冻法操作复杂、费时,所保存的肌腱活性较低。目的:以玻璃化法保存鸡屈趾深肌腱,探索其作为肌腱移植材料的可行性。方法:将成年来亨鸡跖趾关节远侧屈趾深肌腱切断,以数字表法随机分为2组,分别移植同种异体玻璃化肌腱与自体肌腱。术后2,4,8,12,16周观察移植肌腱在形态结构、羟脯氨酸含量和力学性能等方面的变化。结果与结论:玻璃化肌腱移植组早期存在轻度的免疫排斥反应,但不影响肌腱愈合与重建。两组肌腱周围产生中度粘连,移植后肌腱中段的羟脯氨酸含量先减少,8周后逐渐增高,而吻合口部羟脯氨酸含量随时间逐渐增高,两组间羟脯氨酸含量差异无显著性意义。在8周内玻璃化肌腱移植组吻合口部的破裂强度与弹性模量低于自体肌腱移植组(P<0.05),12周后差别无显著性意义。两组肌腱中段部生物力学性能差异无显著性意义。说明玻璃化保存的同种异体肌腱生物活性好、免疫原性低,是良好的肌腱移植材料。

BACKGROUND：The main goal to process tendon allograft was to reduce immunogenicity and to retain structure and activity of tendon at the same time.Cryopreservation was commonly used in clinic,which was complicated,time-consuming,and activity of tendon was lower.OBJECTIVE：To preserve flexor tendon in chicken by vitrification,and to explore its feasibility being used as allograft.METHODS：Far flexor digitorum profundus tendon of adult Leghorn metatarsophalangeal joint was cut off,and randomly divided into two groups with number table：vitrified tendon group and autologous tendon group.Vitrified tendon allograft and autologous tendon was transplanted,respectively.At 2,4,8,12,16 weeks after operation,changes of tendon graft in morphosis,hydroxyproline content and mechanical properties and so on were observed.RESULTS AND CONCLUSION：The vitrified tendon transplantataion group was found light immunologic rejection after implantation in early stage,but which didn＇t affect tendon healing and reconstruction.There was middling level adhesion around tendon in two groups.The content of hydroxyproline in the middle of tendon grafts decreased before 8 weeks,and then gradually increased.However,the content of hydroxyproline in tendon stoma was gradually increased with time.There was no significant difference between two groups.Ruptured intensity and elasticity modulus of the vitrified tendon transplantation group were lower than autologous tendon group（P 0.05） in the part of tendon stoma before 8 weeks,but there was no significant difference after 12 weeks.And there was no significant difference between two groups of the middle of tendon grafts in biomechanics property.The vitrified tendon allograft has good biological viability and low immunogenicity,which could be as well tendon allograft.