锐钛矿型纳米二氧化钛颗粒对人肺腺癌A549细胞的毒性效应

Cytotoxicity of anatase TiO2 nanoparticles on A549 cells

目的：探讨锐钛矿型纳米二氧化钛颗粒（TiO2）对人肺腺癌A549细胞的毒性效应.方法：将不同浓度的锐钛矿型纳米TiO2颗粒悬液与A549细胞共孵育4h后,收集细胞.CCK-8法检测锐钛矿型纳米TiO2颗粒对细胞存活率的影响;采用激光扫描共聚焦显微镜观察锐钛矿型纳米TiO2颗粒进入细胞后活性氧（ROS）的产生状况;采用透射电镜观察锐钛矿型纳米TiO2颗粒引起细胞超微结构的变化.结果：纳米TiO2颗粒对细胞的生长具有抑制作用,且存在剂量-效应关系;染毒后细胞内产生ROS,随着TiO2浓度的增高,ROS的产生增多;透射电镜观察显示纳米TiO2颗粒成簇状存在于细胞中,并对细胞超微结构产生较大损伤.结论：锐钛矿型纳米TiO2颗粒能够诱导细胞产生活性氧,产生细胞毒性,从而损伤细胞超微结构,抑制细胞生长.

Objective： To investigate the toxic effects of anatase TiO2 nanoparticles in vitro. Methods： A549 cells were incubated together with different concentrations of anatase TiO2 nanoparticles for 4 hours, and then collected. CCK-8 assay was performed to evaluate the viability of TiO2-treated cells after a 4-hour exposure. Laser scanning confocal microscope was used to observe the reactive oxygen species （ROS） production after TiO2 nanoparticles entering A549 cells. The cellular ultrastructure changes caused by TiO2 nanoparticles were observed under a transmission electron microscope. Results： The viability of A549 cells was reduced after being exposed to TiO2 in a dose-dependent manner. ROS was observed under a eonfocal microscope, and the levels of ROS had a dose-effect relationship with anatase TiO2 nanoparticles. The distribution of anatase TiO2 nanoparticles was found in cluster in A549 cells under a transmission electron microscope and caused great damage to cell ultrastructure. Conelusion： Anatase TiO2 nanoparticles can result in cytotoxicity in A549 cells through oxidative stress, and thus cause damage to cell ultrastructure and inhibit cell growth.