版纳鱼螈消化道嗜银细胞的分布及形态学

Distribution and morphological characteristics of argyrophilic cells in digestive tract of lchthyophis bannanicus

目的：对我国特有的濒危珍稀两栖动物版纳鱼螈（Ichthyophis bannanicus）消化道嗜银细胞的分布及形态进行研究.方法：取各消化管段,除十二指肠和直肠由于很短全取外,其余的食管、胃、空肠和大肠均分别取始段、中段和末段,应用龙桂开浸银法染色处理,观察嗜银细胞的形态、分布,并测量各消化管段嗜银细胞的分布密度.结果：版纳鱼螈消化道除食管和大肠未见嗜银细胞外,其他部位均有嗜银细胞分布.在胃体和空肠中段有明显的分布密度高峰,其中空肠中段分布密度最高;十二指肠和直肠分布密度较低,以十二指肠分布密度最低;嗜银细胞形态多样,有圆形、锥体形、梭形、椭圆形、柱形和梨形等.结论：版纳鱼螈消化道嗜银细胞的分布特征与中国林蛙（Rana chensinensis）的相似.

Objective： To determine the distribution and morphology of argyrophilic cells in the digestive tract of Ichthyophis bannanicus, which is an endangered Amphibia species in China. Methods： Every segment of the digestive tract was taken. The beginning, middle and end segments of the esophagus, stomach, jejunum and large intestine were taken, except the duodenum and rectum which are too short. Guikai Long silver impregnation method was used to observe the distribution and morphology of the argyrophilic cells, and then the distribution density of the argyrophilic cells in each digestive tract segment was determined. Results： Excluding the esophagus and large intestine, the argyrophil cells were found within the gastrointestinal tract. Among these parts, the argyrophilic cells exhibited its peak distribution within the middle segment of the jejunum, and followed by body of the stomach. Additionally, the argyrophilic cells distributed lower within the rectum and lowest in the duodenum. The appearances of argyrophilie cells varied, including round, cone, spindle, ellipse, column and pear. Conclusion： The distributed feature of argyrophilic cells in the digestive tract of Ichthyophis bannanicus is similar to that of the Rana chensinensis.