硫酸软骨素蛋白多糖4细胞在成年大鼠中枢神经系统的定位分布及其异质性

Localization and heterogeneity of NG2 cells in central nervous system of adult rat

目的：研究硫酸软骨素蛋白多糖4细胞（NG2细胞）在成年大鼠大脑与脊髓的定位分布及其异质性.方法：应用免疫组织化学、免疫荧光方法观察大脑与脊髓NG2阳性细胞分布及其形态特征.采用Image Pro Plus6.0图像分析软件对NG2阳性细胞胞体面积、突起个数、突起长度进行测量.应用SPSS17.0软件对结果进行统计学分析.结果：NG2细胞在成年大鼠中枢神经系统内广泛分布;在脑内NG2细胞突起数目较多,胞体较小;而在脊髓内NG2细胞突起较少,但其胞体却较大.结论：在脑和脊髓内NG2细胞的形态存在异质性,提示其在成年大鼠中枢神经系统两个部位具有不同的功能.

Objective： To observe the localization and heterogeneity of NG2 cells in the brain and spinal cord of adult rats. Methods： The localization and heterogeneity of NG2 cells in the cerebral cortex and spinal cord of adult rats was investigated by using irrmmnohistochemical and immunofluorescence methods. The soma areas, the number of process and the length of process in NG2 cells were measured by image analysis program Image Pro Plus 6.0 and statistically analyzed by SPSS17.0. Results： NG2-positive cells were extensively distributed throughout the CNS of adult rats. The size of NG2 cells in the spi- nal cord was bigger than that in the cerebral cortex; The process length of NG2 cells in the spinal cord was shorter than that in the cerebral cortex; The number of processes in NG2 cells in the cerebral cortex was bigger than that in the spinal cord. Conclusion ： The results indicate that there are some morphological differences between NG2 cells in the spinal cord and NG2 cells in the cerebral cortex. It is suggested that NG2 cells would be heterogeneous in different regions of CNS.