肝门静脉高压症猪肝门静脉的结构重建

Structure remodeling of swine portal vein in portal hypertension

目的：建立猪肝门静脉高压症模型,探讨肝门静脉高压症时肝门静脉的结构重建.方法：猪以四氯化碳、苯巴比妥、乙醇配合高脂、低蛋白、低胆碱饮食进行混合饲养.通过脾静脉插管测压,取门静脉常规石蜡包埋、切片,用H-E 染色法、Weigert法、Aniline blue法、Organge G法分别染组织结构、弹性纤维、胶原纤维和平滑肌,用计算机图像分析系统定量分析肝门静脉的几何形态及显微成分.结果：实验组肝门静脉压为（4.17±1.03）kPa,而正常组为（1.51±0.79）kPa,实验组门静脉的内膜与中膜增厚,管壁增厚,管径增粗,平滑肌、胶原纤维的百分含量增加,平滑肌细胞核的数密度和面密度也在增加,C/E值增加.结论：肝门静脉高压症时,与血液动力学改变相适应,肝门静脉的几何形态与显微结构成分均发生了改变.

Objective： To establish reliable swine portal hypertension model and explore the morphological properties of he- patic portal vein. Methods： Liver cirrhosis and portal hypertension was induced by carbon tetrachloride and fenobarbital （n = 8）, 4 pigs served as the control, and the hepatic arteries were collected and serially sectioned. The tissue structure, elastic fiber, collagen fiber and smooth muscle were stained by H-E, Weigert, Aniline blue and Orange G respectively. The morphological data of hepatic arteries were obtained by the computer image analysis system. Results： The portal vein pressure of the experimental group was （4.17 ±1.03） kPa, and that of normal group was （1.51 ± 0. 79） kPa. The thickness of intima and media wall, diameter of the experimental group were significantly larger than those of the normal group （P 0.01）. The relative content of collagenic fiber and smooth muscle, and C/E of the experimental group were also significantly larger than that of the normal group. Conclusion： In portal hypertension, the structure remodeling of the portal vein was significantly changed, and in return deteriorated the portal hypertension.