HPLC法测定酒石酸长春瑞滨在细胞内外的含量

HPLC method for the determination of vinorelbine tartrate in and out of cells

目的:建立细胞内外酒石酸长春瑞滨含量浓度的高效液相色谱测定方法。方法:色谱柱,LichrospherC18(4.6mm×250mm,5μm);流动相:磷酸盐缓冲液(0.05 mol/ml的磷酸二氢钾,pH 3.5):乙腈=70∶30(v/v);柱温为30℃;检测波长269 nm;流速为1 ml/min;进样体积为10μl。结果:酒石酸长春瑞滨在0.5～25μg/ml范围内线性关系良好,细胞外液的平均提取回收率为84.6%,平均加样回收率为100.3%,日内、日间RSD≤4.84%;细胞内液平均提取回收率为88.2%,平均加样回收率为98.3%,日内、日间RSD≤4.69%;最低检测限0.1μg/ml。结论:本法简单、快速、选择性好,适合酒石酸长春瑞滨含量的测定。

Objective：To establish an HPLC method for the determination of vinorelbine tartrate in and out of cells.Methods：The vinorelbine tartrate was frozen and defrosted time and again.Then the sample was extracted with methanol and determined through Lichrospher C18（4.6 mm×250 mm,5 μm）column in a mobile phase of 50 mmol/ml potassium phosphate buffer（pH 3.5） and acetonitrile（70：30 v/v） with the flow rate of 1.0 ml/min at 30℃.The detection wavelength was 269 nm.Result： It was accurate and sensitive with a linear range from 0.5 μg/ml to 25 μg/ml for Vinorelbine Tartrate.The mean extraction recovery of extracellular fluid obtained was 84.6%,and the absolute recovery was 100.3%.Intra-and inter day periods expressed as percent R.S.D.were≤4.84%.For intracellular fluid,the mean extraction recovery was 88.2% and the absolute recovery was 98.3%.Intra and inter day periods expressed as percent R S D were≤4.69%.The lowest limit of quantification was 0.5 μg/ml.Conclusion：This method is simple,rapid,accurate and selective for the determination of VCR.