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Observation of Insulin Exocytosis by a Pancreatic β Cell Line with Total Internal Reflection Fluorescence Microscopy 被引量：7

Observation of Insulin Exocytosis by a Pancreatic β Cell Line with Total Internal Reflection Fluorescence Microscopy
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摘要 INSULIN secretion was traditionally measured with biochemical and immunological methods such as enzyme linked immunosorbant assay and radioimmunoassay. However, these methods can only tell the amount of insulin secreted; they give no information about the secretion process or mechanism of exocytosis. In recent years, an imaging technique known as total internal reflection fluorescence （TIRF） microscopy has been employed to study insulin secretion.
作者 Zhao-ying Fu Ya-ping Wang Yu Chen
机构地区 College of Medicine Institute of Biophysics
出处 《Chinese Medical Sciences Journal》 CAS CSCD 2011年第1期60-63,共4页 中国医学科学杂志（英文版）
关键词 total internal reflection fluorescence microscopy EXOCYTOSIS INSULIN KISS-AND-RUN 胰岛素分泌 荧光显微镜 胞外分泌 全内反射 细胞系 放射免疫分析法 酶联免疫吸附法 胰腺
分类号 S814.1 [农业科学—畜牧学] TH742.65 [机械工程—光学工程]
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参考文献14

  • 1Fish KN.Total internal reflection fluorescence (TIRF) mi-croscopy.In:Robinson JP,editors.Current protocols in cytometry.New York:John Wiley & Sons Inc.; 2009.p.1801-13.
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同被引文献41

  • 1王金良.关注血清谷氨酰转移酶新的临床应用[J].中华检验医学杂志,2007,30(9):972-973. 被引量：39
  • 2Ludvigsson J,Krisky D, Casas R,et al. GAD65 antigen therapy in recently diagnosed type 1 diabetes mellitus[J]. New England Journal of Medicine, 2012,366 (5) : 433-442.
  • 3Ponsonby AL, Pezic A, Cochrane J, et al. Infant anthropom- etry, early life infection, and subsequent risk of type 1 di- abetes mellitus:a prospective birth cohort study[J]. Pedi- atric Diabetes,2011,12(4ptl ) :313-321.
  • 4De Boer IH,Rue TC,Cleary PA,et al. Long-term renal outcomes of patients with type 1 diabetes mellitus and microalbuminuria:An analysis of the Diabetes Control and Complications Trial/Epidemiology of Diabetes Interven- tions and Complications cohort[J]. Archives of Internal Medicine, 2011,171 (5) : 412-420.
  • 5Skyler JS. Immune intervention for type 1 diabetes melli- tus[J]. International Journal of Clinica! Practice,2011, 65 (s170) :6i-70.
  • 6Alves LI, Davini E, Correia MR,et al. Autoantibodies and high-risk HLA susceptibility markers in first-degree relatives of Brazilian patients with type 1 diabetes mellitus:a progression to disease based study[J]. Journal of Clinical Immunology, 2012,32 (4) : 778-785.
  • 7Youssef D,E1Abbassi A,Woodby G,et al. Benefits of twice-dailyinjection with insulin glargine:A case report and review of the literature[J]. Term Med, 2010,103 (2) : 42-43.
  • 8李宗诚,金在久,王玉宝.月见草油合剂对实验性高脂血症大鼠血脂及血瘀证血液流变学影响的研究[J].中国中医药科技,2008,15(1):30-31. 被引量：4
  • 9贾瑛,汤华.血清胱抑素C和γ-谷氨酰转移酶在糖尿病早期肾损伤的诊断价值[J].陕西医学杂志,2009,38(3):301-302. 被引量：10
  • 10张代民,李娜,张莹.糖尿病患者γ-谷氨酰转移酶的变化及其与血脂的关系[J].国际检验医学杂志,2009,30(8):740-742. 被引量：5

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Chinese Medical Sciences Journal
2011年 第1期

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