尖锐湿疣患者皮损Toll样受体4、9和C型凝集素受体的表达

Expression of Toll-like receptor 4 （TLR4）, TLR9 and DC-specific intraceHular adhesion molecule-3 grabbing non-integrin （SIGN） in condyloma acuminatum lesions

目的探讨初发和复发尖锐湿疣（CA）患者皮损中TLR4、TLR9和DC—SIGN的表达及意义。方法采用免疫组化SP法检测30例初发CA皮损、30例复发CA皮损和20例正常人皮肤（包皮）中TLR4、TLR9和DC—SIGN的表达及分布。结果初发CA皮损和复发CA皮损中TLR4、TLR9和DC—SIGN的表达水平均显著高于正常包皮组织（P〈0．001），且集中分布于基底层和棘层细胞。TLR4、TLR9和DC—SIGN三者在初发CA皮损和复发CA皮损中的表达差异均无统计学意义（三者均P〉0．05）。TLR4、TLR9与DC—SIGN的表达均呈正相关性关系。结论TLR4、TLR9和DC—SIGN的表达增强可能在CA的发病和复发过程中起到一定作用。

Objective To investigate the expression and significance of TLR4, TLR9 and DC-SIGN in primary and recurrent condyloma aeuminatum （CA） lesions. Methods An immunohistochemieal method using streptavidin-peroxidase （SP） was performed to detect the expressions and distribution of TLR4, TLR9 and DCSIGN in tissue specimens obtained from the recurrent CA lesions of 30 patients, primary CA lesions of 30 patients, and from the foreskin of 20 normal human controls. Results The expression levels of TLR4, TLR9 and DCSIGN in primary and recurrent CA lesions were significantly higher than those in normal control tissue （all P 〈 0.001 ）, and the cells expressing TLR4, TLR9 or DC-SIGN were mainly located in the basal and spinous layer in CA lesions. There was no significant difference in the expressions of TLR4, TLR9 or DC-SIGN between primary and recurrent CA lesions （all P 〉 0.05 ）. A positive correlation was found between the expres- sion of TLR4, TLR9 and DC-SIGN in CA lesions. Conclusion The overexpression of TLR4, TLR9 and DCSIGN probably plays an important role in the occurrence and recurrence of CA.