摘要
目的了解全国淋球菌耐药监测点药敏试验的检测水平,分析其影响因素及存往的问题.以提高我国淋球菌耐药监测点实验室药敏试验检测的质量。方法 采用特快专递的方式,将质评样本集中统一发放到全国各淋球菌耐药监测点,由中国疾病预防控制中心性病控制中心将回报结果与预期结果进行比对分析,并将考评结果集中反馈给各参评单位。结果2007-2009年间共发放室间质评考核样本35组,每组3份样本,合计105份,总回报率为88.57%(93/105)。全国有13个淋球菌耐药临测点参加了室间质评活动,3年间参评单位数分别为9、9、13.合格单位数分别为6、7、11。质评考核成绩的总合格率为77.42%(24/31)。淋球菌的药敏检测结果符合率:提高的有质粒介导的产青霉素酶淋球菌、染包体介导的环丙沙星以及大观霉素耐药淋球菌;降低的有质粒介导的高度耐四环素淋球菌和染色体介导的头孢曲松耐药淋球菌结论经过3年的窄间质评,全国淋球菌耐药监测实验事药敏试验的整体检测质量得到了提高,其中产青霉素酶淋球菌、环丙沙星和大观霉素的药敏检测结果的符合率提升较快,而头孢曲松及质粒介导的高度耐四环素淋球菌的药敏检测质量有待提高。
Objective To make a natiomvide external quality assessment fnr drug sensitivity testing of Neisseria gonorrhoeae, analyze the problems in and factors assoeiated with the drug sensitivity testing, and to enhance the quality of drug sensitivity testing of N. gonorrhoeae at different monitoring sites. Methods Samples were uniformly delivered to monitoring sites by express mail service. Test results were analyzed in the National Center for STD Contrail. and the evaluation results were fed back to these monitoring sites. Results A total of 105 quality control samples were delivered from 2007 to 2009, with a response rate of 88.57% (93/105). Thirteen monitoring sites were enrolted in the external quality assessment, including 9 laboratories in 2007. 9 in 2008 and 13 in 2009. The total percentage amounted to 77.42% (24/31) for qualified laboratories during the 3 years, inelnding 6 labnratories in 2007, 7 in 2008 and 11 in 2009. The eoincidence rate increased for the detection of penieillinase-produeing N. gonorrhoeae (PPNG), N. gonotThoeae with ehromosome-me(lialed eiprofloxaein resistance, and N. gonorrhoeae with chromosome-mediated spectinomyein resistance, and declined tor the detection of N. gonorrhoeae with plasmid-mediated high level tetracycline-resistance (THNG) and X. gonorrhoeae with ehromosome-mediated eefiriaxone resistanee. Conclusions The 3-year external quality assessment reveals an improvement in the overall quality of drug sensitivity testing of N. gonor raoeae at national monitoring sites: the aeeuracv is improved markedly for the detection of PPNG, N. gonorrhoeae with resistance to spectinomycin and eiprofloxacin, but is needed taut N. gonorrhoeae and TI/NC.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2011年第5期333-335,共3页
Chinese Journal of Dermatology
关键词
奈瑟球菌
淋病
微生物敏感性试验
质量保证
Neisseria gonorrhoeae
Microbial sensitivity tests
Quality assurance