不同预后尖锐湿疣组织中Toll样受体3、9的表达

Expressions of Toll like receptor 3 （TLR3） and TLR9 in lesions of condyloma acuminatum with different prognosis

目的探讨不同预后尖锐湿疣（CA）患者皮损局部T0u样受体3（TLR3）和Toll样受体9（TLR9）表达定位及TLR3mRNA、TLR9mRNA的表达情况。方法免疫组化SP法及反转录聚合酶链反应（RT—PCR）法检测10例CA复发者、14例CA无复发者及10例包皮组织中TLR3及TLR9表达情况。结果TLR3、TLR9在无复发CA皮损中表达以棘层、颗粒层为主；在复发组CA皮损中表达以基底层、棘层为主。无复发组CA组织中TLR3mRNA表达较对照组及复发组均明显升高（P〈0．01、P〈0．05），TLR9mRNA较对照组明显升高（P〈0．05），较复发组差异无统计学意义。结论表皮TLR3及TLR9的表达部位和表达量的改变可能与CA预后有关，TLR3的影响可能更大。

Objective To investigate the location of TLR3 and TLR9 protein as well as the expressions of TLR3 and TLR9 protein and mRNA in the lesions of condyloma acuminatum （CA） with different prognosis. Methods Immunocytochemical staining with streptavidin-peroxidase （SP） and reverse transcription （RT）-PCR were conducted to detect the expressions of TLR3 and TLR9 in tissue specimens from lesions of 10 patients with recurrent CA, 14 patients with non-recurrent CA as well as from the foreskin of 10 normal human controls. Results TLR3 and TLR9 were mainly distributed in spinous layer and granular layer in non-recur- rent CA lesions, but in basal layer and spinous layer in recurrent CA lesions. A significant elevation was observed in the expression of TLR3 mRNA in non-recurrent CA lesions compared with the control tissue and recurrent CA lesions （P 〈 0.01, 0.05）, and in the expression of TLR9 mRNA in non-recurrent CA lesions compared with the control tissue （P 〈 0.05）. No significant difference was observed between the recurrent and non-recurrent CA lesions in the expression of TLR9 mRNA. Conclusions The changes in the distribution and expressions of TLR3 and TLR9 in epidermis may be associated with the prognosis of CA, and TLR3 may exert a greater impact.