白介素1β抑制培养的大鼠皮层神经元钠电流峰值和动作电位幅度

Interleukin-1β inhibits the amplitudes of voltage-gated Na^+ currents and action potential in cultured cortical neurons of rat

白介素1β(interleukin-1β,IL-1β)是重要的促炎细胞因子,在中枢神经系统的生理学和病理学过程中发挥关键作用。电压门控钠通道是可兴奋细胞电学活动的基础,控制神经元的兴奋性和动作电位。最近的研究又显示了IL-1β与电压门控通道之间的相互作用。为考察中枢神经元中IL-1β与电压门控钠通道之间的相互作用,本研究使用10ng/mL的IL-1β处理培养的大鼠皮层神经元24h,通过电压钳技术测定电压门控钠电流,结果表明IL-1β处理抑制钠电流幅度,但不改变其激活和失活性质。与电压钳记录结果相一致,电流钳记录表明IL-1β降低动作电位幅度但不影响阈值。这些结果显示长时间的IL-1β处理可以抑制电压门控钠电流,这种抑制作用减小了动作电位幅度,这可能改变神经元的电学性质、突触传导等基本功能,并提示了IL-1β在神经系统损伤和疾病中作用的新的思路。

Interleukin-1β （IL-1β） is an important proinflammatory cytokine and plays key roles in physiological and pathophysiological processes of central nervous system（CNS）.The voltage-gated Na＋ channels are essential for electrical properties of excitable cells and control the excitability and action potential（AP） of neurons.Recent studies have showed the relationship between IL-1β and voltage-gated channels.In this work,cultured cortical neurons of rat were treated by 10 ng/mL of IL-1β for 24 h,and then voltage-gated Na＋ currents were recorded using voltage-clamp technique.The results indicated that IL-1β reduced the amplitude of Na＋ currents without any changes in activation or inactivation properties.The current-clamp recording showed that IL-1β reduced the amplitude of AP but not the threshold.These data indicate that IL-1β inhibits the voltage-gated Na＋ currents and the amplitude of AP,and suggest that essential roles of voltage-gated Na＋ channels may be changed by IL-1β.New information about effects of IL-1β on injuries and diseases of CNS was provided.