环境因子对沟金针虫呼吸代谢的影响

Effects of environmental factors on the respiratory metabolism in larvae of Pleonomus canaliculatus(Coleoptera:Elateridae)

为了探讨沟金针虫Pleonomus canaliculatus对不同环境条件的适应性,应用多通道昆虫呼吸仪测定了不同温度、O2浓度、光照强度和湿度条件下沟金针虫的呼吸率。结果显示:沟金针虫的呼吸模式为不规则波动型。温度和光照强度对沟金针虫的CO2释放率影响突出,不同处理间差异显著;不同温度条件下,CO2释放率最小和最大值分别为5℃时的7.22μL/h和35℃时的180.74μL/h,两者间存在极显著差异(P<0.01);强光条件下的沟金针虫呼吸代谢强度大约是无光条件下的2倍(P<0.05)。不同的O2浓度和空气相对湿度对沟金针虫的CO2释放率无明显影响。同时确定了沟金针虫呼吸速率对温度的敏感性——Q10值。结果表明沟金针虫在呼吸代谢过程中对温度和光照反应敏感,对低氧和湿度环境的适应性较强。

In order to understand the adaptability of Pleonomus canaliculatus under different environmental conditions,we detected respiratory rate of insects by using Sable Systems at different temperatures,oxygen concentrations,illumination and humidity. The results indicated that the breathing pattern of P. canaliculatus was the variable-amplitude oscillated type. The CO2 release rate was significantly influenced by temperature and illumination,and there were significant differences between different treatments. The minimum value of the CO2 release rate （7.22 μL/h） was detected at 5℃,while its maximum value was detected （180.74 μL/h） at 35℃,and there was extremely significant difference between the two treatments （P〈0.01）. Respiration intensity of P. canaliculatus under intense light source was two times as high as that under dark （P〈0.05）. The changes of oxygen concentration and air humidity had no significant contribution to the CO2 release rate. The Q10 model which represents the sensitivity of respiration rate of insects to temperature was successfully established by the data analysis. It is so concluded that the temperature and light play a main role in P. canaliculatus respiratory metabolism process whereas low oxygen and humidity are less involved.