摘要
Wolbachia是一类胞质遗传的内共生菌,广泛分布于节肢动物和其他动物中,与宿主的生殖调控密切相关。通过研究迁飞性害虫稻纵卷叶螟Cnaphalocrocis medinalis(Guenée)的Wolbachia感染情况,为探讨Wolbachia在迁飞性昆虫中的生殖调控和传递方式等提供基础资料。本研究应用Wolbachia的ftsZ基因和16S rDNA基因的特异性引物,通过PCR扩增的方法对我国20个地区的稻纵卷叶螟样本进行了检测。结果表明:中国不同地区的稻纵卷叶螟感染Wolbachia的现象较为普遍,其中浙江温州和江苏扬州样本的感染率最高(90%);四川雅安、湖南长沙和天津宁河样本的感染率最低(40%)。不同地区稻纵卷叶螟的Wolbachia ftsZ基因序列完全一致,而且不同地区的Wolbachia 16S rDNA基因序列也完全相同。此外,稻纵卷叶螟感染的Wolbachia ftsZ基因和16S rDNA基因序列与其他物种感染的WolbachiaB群的ftsZ基因序列和16S rDNA基因序列相似性分别在99%～100%和98%～99%之间,说明我国稻纵卷叶螟感染的Wolbachia隶属B群。研究结果表明,稻纵卷叶螟感染的Wolbachia类型较为单一,这也是我国有关稻纵卷叶螟内共生菌Wolbachia的首次研究报道。
Wolbachia is a group of intracellular inherited endosymbiontic bacteria infecting a wide range of arthropods and other animals. The infection status of Wolbachia in the migratory pest Cnaphalocrocis medinalis （Guenée） was studied to provide the basis for revealing the reproductive manipulation mechanism and transmission mechanism of Wolbachia in this pest. In this study,specific primers derived from ftsZ and 16S rDNA genes were used to amplify DNA of Wolbachia from 20 populations of C. medinalis in China by polymerase chain reaction （PCR）. The results indicated that the C. medinalis populations in China were widely infected by Wolbachia,the highest infection rate was 90% in Wenzhou of Zhejiang and Yangzhou of Jiangsu,and the lowest rate was 40% in Ya’an of Sichuan,Changsha of Hunan and Ninghe of Tianjin. The ftsZ sequences and 16S rDNA sequences were exactly the same in all positive samples from different regions. Wolbachia ftsZ sequences and 16S rDNA sequences in C. medinalis showed 99%-100% and 98%-99% similarity with others belonging to Group B,respectively,suggesting that Wolbachia in C. medinalis belong to Group B. The results show that the infection type of Wolbachia in the C. medinalis is relatively single. This is the first report that Wolbachia is distributed in the populations of C. medinalis in China.
出处
《昆虫学报》
CAS
CSCD
北大核心
2011年第4期416-424,共9页
Acta Entomologica Sinica
基金
公益性行业科研专项(200903051)
国家重点基础研究发展规划("973"计划)项目(2006CB102002)
