非小细胞肺癌组织NDRG1表达及其临床意义

Clinical significance of NDRG1 expression in non-small cell lung cancer tissues

目的:分析非小细胞肺癌(NSCLC)组织中N-myc下游调节基因1(NDRG1)的表达及临床意义。方法:免疫组织化学SP和反转录-聚合酶链式反应(RT-PCR)方法分别检测NDRG1在NSCLC组织中的蛋白和mRNA表达。结果:NDRG1的表达定位于细胞质(46/85,54.1%)、细胞核(18/85,21.2%)和细胞膜(9/85,10.6%),总阳性率为61.2%(52/85)。NDRG1在癌旁正常肺组织中的表达(22/85,25.9%)低于肺癌组织,P=0.000。NDRG1在淋巴结转移癌组织中的表达(17/40,42.5%)低于原发灶癌(P=0.003),但高于正常肺组织,P=0.002。NDRG1蛋白表达与肿瘤大小呈正相关,肿瘤≥3 cm组的NDRG1蛋白表达(45/59,76.3%)高于肿瘤<3 cm组(7/26,26.9%),P=0.000。NSCLC组织中NDRG1mRNA水平高于癌旁正常肺组织,P=0.000。NSCLC组织中NDRG1 mRNA表达水平与蛋白表达水平呈正相关,P=0.025。另外,NDRG1阳性组患者的平均生存时间为(35.84±15.07)个月,明显低于NDRG1阴性组患者(63.69±12.16)个月,P=0.002。结论:NSCLC组织中NDRG1的表达相对于癌旁正常肺组织升高,并随肿瘤体积增大而上调,其过表达可能具有提示患者预后不良的意义。

OBJECTIVE： To investigate the expression and clinical significance of NDRG1 in non-small cell lung cancer（NSCLC） tissues.METHODS： The protein and mRNA expression of NDRG1 in NSCLC tissues were examined using immunohistochemistry（IHC） and RT-PCR respectively.RESULTS： NDRG1 expression was found in cytoplasm（46/85,54.1%）,nuclear（18/85,21.2%） and cell membrane（9/85,10.6%） in primary lung cancer tissues with a total positive rate of 61.2%（52/85） which was higher than that in normal lung tissues（22/85,25.9%,P=0.000）.NDRG1 expression in paired lymph node metastases（17/40,42.5%） was lower than that in primary NSCLC（P=0.003） but higher than that in normal lung tissues（P=0.002）.Higher NDRG1 expression was found in NSCLC with large tumor size（≥3 cm）（45/59,76.3% vs 7/26,26.9%,P=0.000）.In addition,NDRG1 mRNA level in primary NSCLC tissues was higher than that in normal lung tissues（P=0.000）.Level of NDRG1 mRNA was correlated with the expression level of NDRG1 protein in primary NSCLC（P=0.025）.Furthermore,the mean survival time of patients with NDRG1-positive tumors（（35.84±15.07） months） was significantly shorter than that of patients with NDRG1-negative tumors（（63.69±12.16） months,P=0.002）.CONCLUSION： The present findings indicate an upregulation of NDRG1 expression during tumor progress which may have prognostic significance for patients with NSCLC.