Duraphat varnish对乳牙釉质氟含量的影响

The effect of Duraphat varnish on fluoride content of enamel in deciduous teeth

目的:检测Duraphat varnish涂布于乳牙后,其釉质内氟元素含量的变化,为临床使用Duraphat varnish预防乳牙龋病提供实验依据。方法:利用电子探针,检测乳牙釉质涂布Duraphat varnish后不同深度的氟含量,并与氟化钠涂布组和生理盐水对照组进行比较;使用X射线光电子能谱仪研究Duraphat varnish涂布剂量及周期变化对乳牙釉质内氟含量的影响。采用SPSS17.0软件包对数据进行统计学分析。结果:对照组、氟化钠组及Duraphat varnish组的氟元素重量百分含量均值分别为(0.0406±0.0234)wt%、(0.1006±0.1040)wt%和(0.1844±0.1293)wt%,Duraphat varnish组氟含量高于氟化钠组,有显著性差异(P<0.01)。Duraphat varnish涂布周期与氟含量关系的实验中,短周期组及长周期组釉质表层下10μm处氟元素重量百分含量均值分别为(0.2407±0.0034)wt%、(0.1434±0.0133)wt%,短周期组氟含量高于长周期组,有显著性差异(P<0.01)。在涂布剂量与氟含量关系的实验中,低剂量组与高剂量组釉质表层下10μm处氟元素重量百分含量均值分别为(0.4417±0.0034)wt%、(0.4413±0.0044)wt%,低剂量组与高剂量组氟含量差别无统计学意义(P>0.05)。结论:乳牙釉质表面局部涂布Duraphat varnish后,釉质中的氟浓度明显升高。缩短该制剂的涂布周期,能有效增加釉质表层内的氟含量,但釉质内氟含量并不随Duraphat varnish涂布剂量增加而升高。

PURPOSE： The aim of this study was to analyse the fluoride distribution in deciduous teeth enamel after using Duraphat varnish,and to supply the evidence for preventing deciduous caries by using Duraphat varnish.METHODS： After coating Duraphat varnish,the alteration of fluoride distribution in the enamel was observed with electron probe micro-analysis.The result was compared with the control group and NaF solution group.The effect of dose and period of coating Duraphat varnish on fluoride distribution in enamel was evaluated by X-ray photo-electron spectrometer（XPS）.The data was analyzed with SPSS17.0 software package.RESULTS： The mean values of fluoride concentration in the control group,NaF group and Duraphat varnish group were（0.0406±0.0234）wt%,（0.1006±0.1040）wt% and（0.1844±0.1293）wt%,respectively.The concentration of fluoride in the Duraphat varnish group was significantly higher than that in the NaF group（P〈0.01）.In period-dependent test,the mean values of fluoride concentration at 10μm under the enamel surface of short period group and long period group were（0.2407±0.0034）wt%,（0.1434±0.0133）wt%;The concentration of fluoride in the short period group was significantly higher than that in the long period group（P〈0.01）.In dose-dependent test,the mean values of fluoride concentration at 10μm under the enamel surface in the low-dose group and high-dose group were（0.4417±0.0034）wt%,（0.4413±0.0044）wt%,the concentration of fluoride in the low-dose group and high-dose group had no significant difference（P〉0.05）.CONCLUSIONS： It is an effective way to increase the concentration of fluoride in enamel by coating Duraphat varnish on the surface of deciduous teeth.Shorten the coating period could increase the concentration of fluoride in enamel effectively but the concentration of fluoride in enamel does not go up along with the coating dose increase.