白斑综合症杆状病毒在养殖万氏对虾(Penaeus vannamei)中暴发流行和人工感染研究

Studies on Epidemiology of White Spot Syndrome Baculovirus(WSBV) in the Culture of Penaeus vannamei and Sensitivity of WSBV in Penaeus vannamei

白斑综合症杆状病毒(White spot syndrome baculovirus,WSBV)对万氏对虾有较强的致病力,在病虾被感染的2～3d内,就可以使万氏对虾死亡率达83.3%～90%.WSBV对万氏对虾的鳃上皮细胞、甲壳下表皮细胞、胃上皮组织细胞、肌细胞、结缔组织细胞和中肠基底膜下肌细胞以及结缔组织细胞敏感.在细胞质中的完整杆状病毒粒子大小为(129～164)nm×(365～412)nm,在细胞核中的完整杆状病毒粒子大小约为(135～176)nm×(429～480)nm.万氏对虾对WSBV没有明显的抗病性,60d养殖死亡率达90%.

Pathoyenicity of white spot syndrome baculovirus to P. vannamei is as strong as to P. monodon . WSBV could infect P. vannamei by feeding, and causeis 83.3%～90% shrimp mortality in 2 ～ 3 days.By using electron microscopy, histology and using PCR, we have identified that the presemce of white spot syndrome baculovirus (WSBV) in the cells of P. vannamei, such as : epithelial cell of gill, cuticle epidermis and stomach tissue cell as well as cell of connective tissuein midgut.