α7烟碱型乙酰胆碱受体对小鼠CD4^+CD25^+调节性T细胞中转录因子STAT5表达的影响

Modulation of STAT5 expression by α7 nicotinic acetylcholine receptor in mouse CD4^+CD25^+ regulatory T cells

目的:检测α7烟碱型乙酰胆碱受体的不同活性状态对CD4+CD25+调节性T细胞中STAT5表达的影响。方法:免疫磁珠法分离C57BL/6J小鼠脾脏中的CD4+CD25+调节性T细胞,以烟碱/α银环蛇毒刺激后采用western blot法分析α7受体的不同活性状态对细胞中STAT5表达的影响。结果:western blot分析显示,α7烟碱型乙酰胆碱受体激动剂--烟碱可以提高调节性T细胞中磷酸化STAT5的表达水平,该效应在给药30min后达峰;而使用α7烟碱型乙酰胆碱受体特异性抑制剂--α银环蛇毒预处理细胞后,烟碱使其中磷酸化STAT5表达增高的效应消失。结论:烟碱可以增强CD4+CD25+调节性T细胞中磷酸化STAT5的表达,且该效应是依赖α7烟碱型乙酰胆碱受体途径的。

Objective：To identify the influence of α7 nicotinic acetylcholine receptor on STAT5 expression in mouse CD4＋CD25＋ regulatory T cells. Method：CD4＋CD25＋ regulatory T cells from the spleen of C57BL/6J mice were separated by means of MACS microbeads,and then we evaluated the STAT5 expression in these cells through western blot test at the present of receptor agonist and antagonist. Result：The findings show that nicotine,an agonist of α7 nicotinic acetylcholine receptor,could upregulate the p-STAT5 expression in regulatory T cells,and this effect peaks 30 minutes after drug administration. Pretreated cells with α7 nicotinic acetylcholine receptor＇s specific antagonist α-bungarotoxin,nicotine will no longer affect the p-STAT5 expression. Conclusion：Nicotine could enhance the expression of p-STAT5 in mouse CD4＋CD25＋ regulatory T cells,and this effect depends on the α7 nicotinic acetylcholine receptor pathway.