北京地区百合无症病毒CP基因的克隆与分析

Clone and analysis of gene CP of Lily Symptomless virus in Beijing area

以感染百合无症病毒(LSV)的百合叶片为材料,提取总RNA为模板,通过反转录聚合酶链式反应(RT-PCR)扩增出856bp大小的LSV CP基因片段。经Blast比对发现,该基因片段与Genbank上发表的LSV CP基因序列(DQ294655.1)同源性为97.31%。由该序列推导出的氨基酸序列与其相似度为98.6%,仅存在某些氨基酸的差异。经过聚类分析表明,该CP基因的氨基酸序列与厦门和兰州分离的病毒遗传距离较近,而与海宁和广州分离的病毒遗传距离较远。

A CP cDNA sequence of 856 bp fragment of Lily Symptom virus（LSV） gene was amplified by reverse transcription polymerase chain reaction（RT-PCR） from the total RNA extracted from infected lily leaves.The blast results showed that the sequence presented a very high match with the LSV gene CP in the GenBank（DQ294655.1） by 97.31% homology,the similarity of the amino acid pushed out by this sequence was 98.6% with only a few differences in amino acids.According to cluster analysis results,the genetic distance of the amino acid sequence of gene CP isolated from Xiamen was closed to that from xiamen and Lanzhou,and the genetic distance of it was far from that from Haining and Guangzhou.