创伤愈合过程中酸性粘多糖的定位分布

Distribution of acid mucopolysaccharides in wound healing.

观察胚胎兔、成兔皮肤在正常发育及创伤愈合过程中羧基、硫酸基粘多糖的定位分布。利用胎兔创伤模型，切取皮肤组织标本，用Ｓｐｉｃｅｒ的特殊染色方法切片观察。随胎兔不断成熟，表皮层中除基底层外羧基粘多糖逐渐减少，真皮层呈一致弥漫的分布，成兔表皮层主要为硫酸基粘多糖，真皮网状层的羧粘多糖多围绕胶原纤维分布，密度较深，两者羧基粘多糖染色最浓的部位位于基底层附近。创伤后，胎及成兔均表现出表皮基底层及真皮层羧基粘多糖合成增加，两者的差别不甚显著，而胎兔则在创伤后第５天完全愈合。硫酸基粘多糖的分布部位主要在表皮浅层和真皮层的毛囊细胞、血管及腺体上皮之中，创伤后反应不明显。羧基粘多糖是参与创伤愈合反应的主要细胞外基质，在创伤愈合及瘢痕形成领域具有十分重要的作用。

By excising normal and wounded skin and using spicer′s dyeing method on a wound healing model of rabbit fetal we observed the distribution of acid mucopolysaccharides of fetal and adult rabbit skin in normal development and wound healing. The most heavy dyeing lay in the basal layer of epidermis whether in fetus or in adult rabbit. Other layers in epidermis became less and less dyeing of carboxyl mucopolysaccharides with development of the fetus, while in the dermis there was conslltantly heavy dyeing. There were only sulfate mucopolysaccharides present in epidermis in adult, carboxyl mucopolysaccharides in dermis were distributed around the collagen fibers and dyeing more heavier. Carboxyl mucopolysaccharides dyeing became heavier after wounding both in fetus and adults with no difference between them. The carboxyl mucopolysaccharides is the major extracellular matrix involved and plays important role in wound healing and scar formation.